Introduction: Human Immunodeficiency Virus (HIV) infection and malnutrition are interlinked, both epidemiologically and physiologically. The synergistic effects of malnutrition and HIV on the immune system occur in a vicious cycle in which the decreased immunity associated with both conditions leads to increased susceptibility to infections which if not adequately met lead to more malnutrition. As a result, there has been the development and implementation of guidelines on how best to offer nutritional care to HIV-infected children. Objectives: To Determine the Knowledge and Practices of Healthcare workers at Kenyatta National Hospital as regards the nutritional assessment, nutritional classification and indicators of increased nutritional needs in HIV infected children.