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Non-Communicable diseases (NCDs) are the leading cause of morbidity and mortality in many developed and developing countries. Nigeria is experiencing an epidemiological transition with a double burden of communicable and non-communicable diseases. This study was carried out to assess the knowledge, attitude and practice of bankers in Rivers State on healthy lifestyle in relation to the prevention and control of NCDs. A descriptive cross-sectional design was employed. Multistage sampling technique was used to collect information from 800 consenting bankers using pretested, interviewer-administered questionnaires. The questionnaire collected information on biodata, knowledge, attitude and practice. Data obtained was analyzed using statistical packages for service solution (SPSS) version 25. The result revealed a mean age for the 800 respondents as 33.5 ± 5.7 years. Only 26.9% had good knowledge of a healthy lifestyle while 87.3% had a good attitude towards a healthy lifestyle. Only9.7% consumed a healthy diet, while the majority 88.0% and 72.7% had good practice regarding tobacco smoking and alcohol consumption respectively.