Early neonatal death is often due to prenatal events such as severe prenatal asphyxia, infections and pre-maturity and is a major contributor to infant mortality. Poor resuscitation due to incompetent health care provider contributed significantly to this asphyxia related mortality and morbidity. The aim of this study is to assess knowledge, practice & associated factors toward neonatal resuscitation among nurses and MW in South Wollo public hospitals, Ethiopia. A cross-sectional study design employed in a total of 143 participants, from this only 32.9% and 24.5% of the participants had good knowledge and practice towards resuscitation respectively. Lack of training and lack of supportive supervision were significantly associated with poor knowledge score, whereas absence of guidelines and lack of supportive supervision were significantly associated with poor performance of the participants. Therefore, training was found to be a single predictor for knowledge while supportive supervision and guideline were predictors for knowledge and practice. Continuous and regular training and supportive supervision for nurses and MS should be strengthening.