L-methioninase production by Aspergillus flavipes from Sesame oil cake - Swathi, A.;Sridevi, V.
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Solid-state fermentation was carried out for the production of extra-cellular L-methioninase by Aspergillus flavipes MTCC 6337 using ten agro-industrial residues. Sesame oil cake was selected as solid substrate for L-methioninase production by A. flavipes. The maximum L-methioninase yield (396 U/gds) was obtained in preliminary studies by incubating flasks containing sesame oil cake of 60% initial moisture content (pH 8.0) as substrate supplemented with D-glucose (1.0% w/v), L-methionine (0.8% w/v) , MnSO4 (0.5% w/v) and 3 ml of A. flavipes conidial suspension for 8 days at 30 0C. 4 parameters…mehr

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Produktbeschreibung
Solid-state fermentation was carried out for the production of extra-cellular L-methioninase by Aspergillus flavipes MTCC 6337 using ten agro-industrial residues. Sesame oil cake was selected as solid substrate for L-methioninase production by A. flavipes. The maximum L-methioninase yield (396 U/gds) was obtained in preliminary studies by incubating flasks containing sesame oil cake of 60% initial moisture content (pH 8.0) as substrate supplemented with D-glucose (1.0% w/v), L-methionine (0.8% w/v) , MnSO4 (0.5% w/v) and 3 ml of A. flavipes conidial suspension for 8 days at 30 0C. 4 parameters were selected for optimization by Central Composite Design (CCD) which showed significant effect on L-methioninase production in the preliminary studies. They include inoculum volume, initial moisture content, carbon supplement concentration and pH. A second order polynomial model equation was obtained. The predicted optimum levels were as follows: inoculum volume - 3.0814 ml, initial moisture content - 62.19003% (v/w), carbon supplement concentration - 0.9995% (w/v) and pH - 8.36124.The enzyme L-methioninase was partially purified using 70% saturated ammonium sulphate followed by dialysis.
Autorenporträt
Miss. A. Swathi is pursuing M.Tech Biotechnology in Andhra University College of Engineering (A). Her project area includes fermentation. She has Published papers in 5 reputed International journals. She has attended 10 national and 2 international conferences. She has been awarded gold medal for B.Tech Biotechnology from ANITS , Visakhapatnam.