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This book is designed for to learn the strategies of biochemical research,to provide ample opportunity for the students to specialize in basic and advanced methods used in investigation focusing on biology applications.To learn qualitative and quantitative phytochemicals analysis -Alkaloids,Flavanoids,Steroids ,Tannins,Saponins.Antibacterial activity by disc diffusion method,In vitro antioxidant activity,Estimation of soil mineral content-pH,nitrate, nitrite, sulphate, phosphate, calcium, magnesium,chloride, fluoride, silica and ammonia. Widal test, VDRL test, Immunoelectrophoresis,Skin prick test.