This practical laboratory manual has been designed to provide the exposure to students with methods and protocols in the field of Industrial Biotechnology, Environmental Biotechnology, Genomics & Proteomics and Computational Biology. It is a complete instructional manual that imparts knowledge on principle, procedure, observations and conclusions. The fundamentals in this manual are critical to the success of undergraduate /post-graduate students and research scholars of Biotechnology. The manual imparts students with the basic laboratory skills and knowledge to pursue a career in Biotechnology. These exercises and activities serve to engage students and help them understand the fundamentals of working in a biotechnology laboratory. Building students' skills through an organised and systemic presentation of materials & methods and tasks, the manual will help students to explore overarching themes that relate to all domains of biotechnology. Book is recommended for B.Sc., B.Tech.,M.Sc., M.Tech. students & Ph.D. scholars of Biotechnology.