Laboratory Applications in Microbiology: A Case Study Approach includes a photo atlas with more than 250 full-color images! This lab uses real-life case studies as the basis for exercises in the laboratory. This is the only microbiology lab manual focusing on this means of instruction, an approach particularly applicable to the microbiology laboratory. The author has carefully organized the exercises so that students develop a solid intellectual base beginning with a particular technique, moving through the case study, and finally applying new knowledge to unique situations beyond the case study.…mehr
Laboratory Applications in Microbiology: A Case Study Approach includes a photo atlas with more than 250 full-color images! This lab uses real-life case studies as the basis for exercises in the laboratory. This is the only microbiology lab manual focusing on this means of instruction, an approach particularly applicable to the microbiology laboratory. The author has carefully organized the exercises so that students develop a solid intellectual base beginning with a particular technique, moving through the case study, and finally applying new knowledge to unique situations beyond the case study.Hinweis: Dieser Artikel kann nur an eine deutsche Lieferadresse ausgeliefert werden.
Barry Chess has been teaching microbiology at Pasadena City College for over 15 years. He received his Bachelor's and Master's degrees from California State University, Los Angeles, and did several years of postgraduate work at the University of California, Irvine, where his research focused on the expression of eukaryotic genes involved in the development of muscle and bone. At Pasadena City College, Barry developed a new course in human genetics and helped to institute a biotechnology program. He regularly teaches courses in microbiology, general biology, and genetics, and works with students completing independent research projects in biology and microbiology. Over the past several years, Barry's interests have begun to focus on innovative methods of teaching that lead to greater student understanding. He has written cases for the National Center for Case Study Teaching in Science and presented talks at national meetings on the use of case studies in the classroom. In 2009, his laboratory manual, Laboratory Applications in Microbiology: A Case Study Approach, was published. He is thrilled and feels very fortunate to be collaborating with Kathy Talaro, with whom he has worked in the classroom for more than a decade, on this ninth edition. Barry is a member of the American Society for Microbiology and regularly attends meetings in his fields of interest, both to keep current of changes in the discipline and to exchange teaching and learning strategies with others in the field.
Inhaltsangabe
1 Safety Considerations in the Microbiology Laboratory 2 Microscopy and Measurement of Microscopic Specimens 3 A Survey of Protists 4 A Survey of Fungi 5 A Survey of Parasitic Worms 6 Ubiquity of Microorganisms 7 Aseptic and Pure Culture Techniques 8 Simple Staining, Negative Staining, and Gram Staining 9 Capsular Staining 10 Endospore Staining 11 Acid-Fast Staining 12 Viable Plate Count 13 Cultivation of Anaerobes 14 Temperature Effects on Bacterial Growth and Survival 15 pH and Microbial Growth 16 Effects of Osmotic Pressure on Bacterial Growth 17 Lethal Effects of Ultraviolet Light 18 Evaluation of Disinfectants 19 Effectiveness of Hand Scrubbing 20 Antimicrobic Sensitivity Testing: Kirby-Bauer, Tube Dilution, and ETEST(C) Methods 21 Simulated Epidemic 22 Morbidity and Mortality Weekly Report 23 Bacterial Transformation 24 The Ames Test 25 DNA Extraction from Bacterial Cells 26 DNA Profiling 27 Blood Typing 28 Rapid Identification of Staphylococcus aureus Using Latex Agglutination Testing 29 Slide Agglutination 30 Enzyme-Linked Immunosorbent Assay (ELISA) 31 Biofilm Culture and Examination 32 Measures of Water Quality: Most Probable Number Procedure 33 Measures of Water Quality: Membrane Filtration Method 34 Measures of Milk Quality: Methylene Blue Reductase Test 35 Bacterial Counts of Food 36 Isolation and Identification of Staphylococci 37 Isolation and Identification of Streptococci 38 Epidemiology of Gastrointestinal Illness: Differentiation of Enterobacterlaceae 39 Differential White Blood Cell Count 40 Identification of Bacterial Unknowns 41 Colony Morphology 42 Growth in Solid and Liquid Media 43 Motilty Methods: Wet Mount and Hanging Drop 44 Flagella Stain 45 Streak-Plate Isolation 46 Loop Dilution 47 Spread-Plate 48 Fluid Thioglycollate Medium 49 CHROMagar Orientation Medium 50 Mannitol Salt Agar 51 MacConkey Agar 52 Desoxycholate Agar 53 Endo Agar 54 Eosin Methylene Blue Agar 55 Hektoen Enteric Agar 56 Xylose Lysine Desoxycholate Agar 57 Blood Agar 58 Motility Medium 59 SIM Medium 60 Kligler's Iron Agar 61 Triple Sugar Iron Agar 62 Lysine Iron Agar 63 Litmus Milk 64 Oxidation-Fermentation Test 65 Phenol Red Carbohydrate Broth 66 Purple Carbohydrate Broth 67 Methyl Red and Voges-ProskauerTests 68 Catalase Test 69 Oxidase Test 70 Nitrate Reduction Test 71 Coagulase Test 72 Citrate Test 73 Malonate Test 74 Amino Acid Decarboxylation Test 75 Phenylalanine Deaminase Test 76 Bile Esculin Test 77 Starch Hydrolysis 78 ONPG Test 79 Urease Test 80 Casease Test 81 Gelatinase Test 82 DNase Test 83 Lipase Test 84 CAMP Test 85 PYR Test 86 API 20E System 87 EnteroPluri-Test System 88 Antibiotic Disk Sensitivity Tests 89 B-Lactamase Test 90 Viable Plate Count 91 Direct Cell Count
1 Safety Considerations in the Microbiology Laboratory 2 Microscopy and Measurement of Microscopic Specimens 3 A Survey of Protists 4 A Survey of Fungi 5 A Survey of Parasitic Worms 6 Ubiquity of Microorganisms 7 Aseptic and Pure Culture Techniques 8 Simple Staining, Negative Staining, and Gram Staining 9 Capsular Staining 10 Endospore Staining 11 Acid-Fast Staining 12 Viable Plate Count 13 Cultivation of Anaerobes 14 Temperature Effects on Bacterial Growth and Survival 15 pH and Microbial Growth 16 Effects of Osmotic Pressure on Bacterial Growth 17 Lethal Effects of Ultraviolet Light 18 Evaluation of Disinfectants 19 Effectiveness of Hand Scrubbing 20 Antimicrobic Sensitivity Testing: Kirby-Bauer, Tube Dilution, and ETEST(C) Methods 21 Simulated Epidemic 22 Morbidity and Mortality Weekly Report 23 Bacterial Transformation 24 The Ames Test 25 DNA Extraction from Bacterial Cells 26 DNA Profiling 27 Blood Typing 28 Rapid Identification of Staphylococcus aureus Using Latex Agglutination Testing 29 Slide Agglutination 30 Enzyme-Linked Immunosorbent Assay (ELISA) 31 Biofilm Culture and Examination 32 Measures of Water Quality: Most Probable Number Procedure 33 Measures of Water Quality: Membrane Filtration Method 34 Measures of Milk Quality: Methylene Blue Reductase Test 35 Bacterial Counts of Food 36 Isolation and Identification of Staphylococci 37 Isolation and Identification of Streptococci 38 Epidemiology of Gastrointestinal Illness: Differentiation of Enterobacterlaceae 39 Differential White Blood Cell Count 40 Identification of Bacterial Unknowns 41 Colony Morphology 42 Growth in Solid and Liquid Media 43 Motilty Methods: Wet Mount and Hanging Drop 44 Flagella Stain 45 Streak-Plate Isolation 46 Loop Dilution 47 Spread-Plate 48 Fluid Thioglycollate Medium 49 CHROMagar Orientation Medium 50 Mannitol Salt Agar 51 MacConkey Agar 52 Desoxycholate Agar 53 Endo Agar 54 Eosin Methylene Blue Agar 55 Hektoen Enteric Agar 56 Xylose Lysine Desoxycholate Agar 57 Blood Agar 58 Motility Medium 59 SIM Medium 60 Kligler's Iron Agar 61 Triple Sugar Iron Agar 62 Lysine Iron Agar 63 Litmus Milk 64 Oxidation-Fermentation Test 65 Phenol Red Carbohydrate Broth 66 Purple Carbohydrate Broth 67 Methyl Red and Voges-ProskauerTests 68 Catalase Test 69 Oxidase Test 70 Nitrate Reduction Test 71 Coagulase Test 72 Citrate Test 73 Malonate Test 74 Amino Acid Decarboxylation Test 75 Phenylalanine Deaminase Test 76 Bile Esculin Test 77 Starch Hydrolysis 78 ONPG Test 79 Urease Test 80 Casease Test 81 Gelatinase Test 82 DNase Test 83 Lipase Test 84 CAMP Test 85 PYR Test 86 API 20E System 87 EnteroPluri-Test System 88 Antibiotic Disk Sensitivity Tests 89 B-Lactamase Test 90 Viable Plate Count 91 Direct Cell Count
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