For general microbiology laboratory courses Laboratory Experiments in Microbiology features 57 thoroughly class-tested and easily customizable exercises that teach basic microbiology techniques and applications. The manual provides comprehensive coverage of every area of microbiology for undergraduate students across diverse disciplines, including the biological sciences, allied health sciences, agriculture, environmental science, nutrition, pharmacy, and various pre-professional programs. The lab manual is the perfect companion to Tortora/Funke/Case’s Microbiology: An Introduction, 13th…mehr
For general microbiology laboratory courses Laboratory Experiments in Microbiology features 57 thoroughly class-tested and easily customizable exercises that teach basic microbiology techniques and applications. The manual provides comprehensive coverage of every area of microbiology for undergraduate students across diverse disciplines, including the biological sciences, allied health sciences, agriculture, environmental science, nutrition, pharmacy, and various pre-professional programs. The lab manual is the perfect companion to Tortora/Funke/Case’s Microbiology: An Introduction, 13th Edition or any introductory microbiology text. The 12th Edition of Laboratory Experiments in Microbiology is easier than ever to navigate and more visually effective with new icons indicating when an exercise addresses the human or environmental microbiome, is investigative, or addresses an ASM guideline. New ASM Thinking Skills outline the steps that help students develop laboratory thinking skills. Pre-lab quizzes in Mastering™ Microbiology ensure students arrive prepared for each lab, and activities such as Lab and Lecture: Putting It All Together help students see how lab and lecture are integrated. Hinweis: Dieser Artikel kann nur an eine deutsche Lieferadresse ausgeliefert werden.
About our authors Ted R. Johnson is a professor emeritus of biology at St. Olaf College, a liberal arts college in Northfield, Minnesota, where he has taught courses in microbiology and immunology. He has taught at St. Olaf for 39 years and previously taught at Mankato State University for five years. He received his master’s degree and his Ph.D in microbiology from the University of Illinois in Chicago, Illinois. While at St. Olaf College, he has developed and directed several semester abroad and interim programs. He has been involved in advising health professions students and received the Fr.Joseph Walter Award for Health Profession advising. His research focuses on the immune response to virally induced cancer in various animal models. Christine L. Case is a professor of microbiology at Skyline College in San Bruno, California, where she has taught for the past 47 years. She received her Ed. D. in curriculum and instruction from Nova Southeastern University and her M.A. in microbiology from San Francisco State University. She was Director for the Society for Industrial Microbiology (SIM). She received the ASM and California Hayward outstanding educator awards and SACNAS Mentor award. In addition to teaching, Chris contributes regularly to professional literature, develops innovative educational methodologies, and maintains a personal and professional commitment to conservation and the importance of science in society. Chris is also an avid photographer, and many of her photographs appear in this lab manual.
Inhaltsangabe
PART ONE MICROSCOPY 1. Use and Care of the Microscope 2. Examination of Living Microorganisms PART TWO HANDLING BACTERIA 1. Microbes in the Environment 2. Transfer of Bacteria: Aseptic Technique PART THREE STAINING METHODS 1. Preparation of Smears and Simple Staining 2. Negative Staining 3. Gram Staining 4. Acid-Fast Staining 5. Structural Stains (Endospore, Capsule, and Flagella) 6. Morphological Unknown PART FOUR CULTIVATION OF BACTERIA 1. Isolation of Bacteria by Dilution Techniques 2. Special Media for Isolating Bacteria PART FIVE MICROBIAL METABOLISM 1. Carbohydrate Catabolism 2. Fermentation 3. Protein Catabolism, Part 1 4. Protein Catabolism, Part 2 5. Respiration 6. Unknown Identification and Bergeys Manual PART SIX MICROBIAL GROWTH 1. Oxygen and the Growth of Bacteria 2. Determination of a Bacterial Growth Curve: The Role of Temperature 3. Biofilms PART SEVEN CONTROL OF MICROBIAL GROWTH 1. Physical Methods of Control: Heat 2. Physical Methods of Control: Ultraviolet Radiation 3. Chemical Methods of Control: Disinfectants and Antiseptics 4. Chemical Methods of Control: Antimicrobial Drugs 5. Effectiveness of Hand Scrubbing PART EIGHT MICROBIAL GENETICS 1. Regulation of Gene Expression 2. Isolation of Bacterial Mutants 3. Transformation of Bacteria 4. DNA Fingerprinting 5. Genetic Engineering 6. Ames Test for Detecting Possible Chemical Carcinogens PART NINE THE MICROBIAL WORLD 1. Fungi: Yeasts and Molds 2. Phototrophs: Algae and Cyanobacteria 3. Protozoa 4. Parasitic Helminths PART TEN VIRUSES 1. Isolation and Titration of Bacteriophages 2. Plant Viruses PART ELEVEN INTERACTION OF MICROBE AND HOST 1. Epidemiology 2. Koch's Postulate PART TWELVE IMMUNOLOGY 1. Innate Immunity 2. Agglutination Reactions: Slide Agglutination 3. Agglutination Reactions: Microtiter Agglutination 4. ELISA Technique PART THIRTEEN MICRORGANISMS AND DISEASE 1. Bacteria of the Skin 2. Bacteria of the Respiratory Tract 3. Bacteria of the Mouth 4. Bacteria of the Gastrointestinal Tract 5. Bacteria of the Urogenital Trac 6. Identification of an Unknown from a Clinical Sample 7. Rapid Identification Methods PART FOURTEEN MICROBIOLOGY AND THE ENVIRONMENT 1. Microbes in Water: Multiple-Tube Technique 2. Microbes in Water: Membrane Filter Technique 3. Microbes in Food: Contamination 4. Microbes Used in the Production of Foods 5. Microbes in Soil: The Nitrogen and Sulfur Cycles 6. Microbes in Soil: Bioremediation APPENDICES * A. Pipetting * B. Dilution Techniques and Calculations * C. Use of the Spectrophotometer * D. Graphing * E. Use of the Dissecting Microscope * F. Use of the Membrane Filter * G. Electrophoresis * H. Keys to Bacteria
PART ONE MICROSCOPY 1. Use and Care of the Microscope 2. Examination of Living Microorganisms PART TWO HANDLING BACTERIA 1. Microbes in the Environment 2. Transfer of Bacteria: Aseptic Technique PART THREE STAINING METHODS 1. Preparation of Smears and Simple Staining 2. Negative Staining 3. Gram Staining 4. Acid-Fast Staining 5. Structural Stains (Endospore, Capsule, and Flagella) 6. Morphological Unknown PART FOUR CULTIVATION OF BACTERIA 1. Isolation of Bacteria by Dilution Techniques 2. Special Media for Isolating Bacteria PART FIVE MICROBIAL METABOLISM 1. Carbohydrate Catabolism 2. Fermentation 3. Protein Catabolism, Part 1 4. Protein Catabolism, Part 2 5. Respiration 6. Unknown Identification and Bergeys Manual PART SIX MICROBIAL GROWTH 1. Oxygen and the Growth of Bacteria 2. Determination of a Bacterial Growth Curve: The Role of Temperature 3. Biofilms PART SEVEN CONTROL OF MICROBIAL GROWTH 1. Physical Methods of Control: Heat 2. Physical Methods of Control: Ultraviolet Radiation 3. Chemical Methods of Control: Disinfectants and Antiseptics 4. Chemical Methods of Control: Antimicrobial Drugs 5. Effectiveness of Hand Scrubbing PART EIGHT MICROBIAL GENETICS 1. Regulation of Gene Expression 2. Isolation of Bacterial Mutants 3. Transformation of Bacteria 4. DNA Fingerprinting 5. Genetic Engineering 6. Ames Test for Detecting Possible Chemical Carcinogens PART NINE THE MICROBIAL WORLD 1. Fungi: Yeasts and Molds 2. Phototrophs: Algae and Cyanobacteria 3. Protozoa 4. Parasitic Helminths PART TEN VIRUSES 1. Isolation and Titration of Bacteriophages 2. Plant Viruses PART ELEVEN INTERACTION OF MICROBE AND HOST 1. Epidemiology 2. Koch's Postulate PART TWELVE IMMUNOLOGY 1. Innate Immunity 2. Agglutination Reactions: Slide Agglutination 3. Agglutination Reactions: Microtiter Agglutination 4. ELISA Technique PART THIRTEEN MICRORGANISMS AND DISEASE 1. Bacteria of the Skin 2. Bacteria of the Respiratory Tract 3. Bacteria of the Mouth 4. Bacteria of the Gastrointestinal Tract 5. Bacteria of the Urogenital Trac 6. Identification of an Unknown from a Clinical Sample 7. Rapid Identification Methods PART FOURTEEN MICROBIOLOGY AND THE ENVIRONMENT 1. Microbes in Water: Multiple-Tube Technique 2. Microbes in Water: Membrane Filter Technique 3. Microbes in Food: Contamination 4. Microbes Used in the Production of Foods 5. Microbes in Soil: The Nitrogen and Sulfur Cycles 6. Microbes in Soil: Bioremediation APPENDICES * A. Pipetting * B. Dilution Techniques and Calculations * C. Use of the Spectrophotometer * D. Graphing * E. Use of the Dissecting Microscope * F. Use of the Membrane Filter * G. Electrophoresis * H. Keys to Bacteria
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