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This Biochemistry laboratory manual is a compilation of laboratory procedures involving colorimetric estimations of Creatinine, Urea, DNA & RNA, Cholesterol and Glucose. This manual includes enzyme assays of Urease, Salivary amylase and their optimization procedures. The Urinary analysis procedure contains qualitative analysis of normal and abnormal Urine constituents and their clinical significance. Haematology manual includes the determination of blood groups and Rh factor, Haemoglobin, Erythrocyte Sedimentation Rate, RBC & WBC, Differential counting and Blood pressure. This manual will be useful for undergraduate and postgraduate college students of Biochemistry discipline.