Plant viruses cause major losses to several agricultural and horticultural crops around the world. The present century faces severe epidemics by newly emerging viruses affecting a variety of crops, particularly vegetables, legumes, grains, oil seeds, fruits, tuber crops, spices and ornamental plants. This text book has covered basics of plant viruses like general features, brief history, classification, some economically important plant viruses, its symptoms, transmission study and various protocols (biological, serological and molecular) for detection, identification and characterization of plant viruses that can be applied to the management of plant virus diseases and improve the quality and yield of crop plants. The Laboratory Manual of Plant Virology book guide is intended for the use in detection, identification and characterization of various groups of plant viruses on economically important plants. This book also help to PG students, research scholars and faculty members to handle the plant virus in laboratory, in the field and green houses and identified them at biological, serological and molecular levels.