This laboratory manual provides up-to-date and comprehensive details of analytical methods of fish parasite and antibiotic residues assay in reference to fishes. Several antibiotics, anaesthetics are being used in culture system now days as antibacterial agent. However, high dosage and improper use of antibiotics have resulted in levels of antibiotic residues that are higher than permitted, raising concern over the effects of antibiotics on the environment and on consumer health. This manual will be useful for fishery professionals, scientists, and students as a useful analytical methods in general and impart significant knowledge on fish parasitological and pharmacology to the students community in specific.