Cell biology spans among the widest diversity of methods in the biological sciences. From physical chemistry to microscopy, cells have given up with secrets only when the questions are asked in the right way! This new volume of Methods in Cell Biology covers laboratory methods in cell biology and includes methods that are among the most important and elucidating in the discipline, such as bioluminescent imaging of gene expressions, confocal imaging, and electron microscopy of bone.
Cell biology spans among the widest diversity of methods in the biological sciences. From physical chemistry to microscopy, cells have given up with secrets only when the questions are asked in the right way! This new volume of Methods in Cell Biology covers laboratory methods in cell biology and includes methods that are among the most important and elucidating in the discipline, such as bioluminescent imaging of gene expressions, confocal imaging, and electron microscopy of bone.
1. Single Molecule FRET Microscopy 2. Bioluminescent Imaging of Gene Expressions 3. Confocal Imaging 4. Electron Microscopy of Bone 5. Immunostaining: Detection of Signaling Protein Location in Tissues, Cells and Subcellular Compartments 6. Bimolecular Fluorescence Complementation (BiFC) Analysis of Protein-Protein Interactions 7. Histochemistry 8. Live Imaging 9. Click Labeling for Single-Molecule FRET 10. High Throughput Methods for the Detection of Protein Over Expression Using Fluorescent Markers 11. Correlation Microscopy 12. Stereology of the Lung 13. Formation and Preparation of Cellular Spheroids for High-Content Assays Based on Epifluorescence and Laser Light Sheet Fluorescent Microscopies. 14. Optimising Fluorochrome Excitation and Detection for Intravital 2-Photon Microscopy 15. Cryomicroscopy 16. In situ Hybridization 17. Imaging RNA
1. Single Molecule FRET Microscopy 2. Bioluminescent Imaging of Gene Expressions 3. Confocal Imaging 4. Electron Microscopy of Bone 5. Immunostaining: Detection of Signaling Protein Location in Tissues, Cells and Subcellular Compartments 6. Bimolecular Fluorescence Complementation (BiFC) Analysis of Protein-Protein Interactions 7. Histochemistry 8. Live Imaging 9. Click Labeling for Single-Molecule FRET 10. High Throughput Methods for the Detection of Protein Over Expression Using Fluorescent Markers 11. Correlation Microscopy 12. Stereology of the Lung 13. Formation and Preparation of Cellular Spheroids for High-Content Assays Based on Epifluorescence and Laser Light Sheet Fluorescent Microscopies. 14. Optimising Fluorochrome Excitation and Detection for Intravital 2-Photon Microscopy 15. Cryomicroscopy 16. In situ Hybridization 17. Imaging RNA
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