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The objective of this study is to evaluate the efficacy of low-level diode laser in soft tissue wound healing. The current study includes experimental and clinical parts. The experimental study was performed on seven healthy local bred rabbits. Four incisions were done in each side at dorsal surface for each rabbit. The wounds at the right side were irradiated by low level diode laser while the wounds at left side were left without irradiation as a control. Animals were divided into two groups according to laser parameters: 1- Group A includes three rabbits and the wounds were irradiated by 1.25W/cm2 power density, 20 sec. exposure time. Clinical study was performed on 20 patients 10 male, 10 female , age range between 5-75 years with oral and maxillofacial lesions. After surgical intervention was performed, the wounds were divided into two parts, one part was irradiated by 1.25 W/cm2 , 50 sec. low level diode laser. Other part was left as a control.