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This volume comprises a collection of invited and selected contributions presented at the 16th International Conference on Laser Spectroscopy in Palm Cove, Queensland, Australia, 13-18 July 2003. The papers report the latest and most exciting developments in laser spectroscopy and related areas: new ultraprecise spectroscopic measurements based on optical frequency combs including tests of the stability of the fundamental constants; the first successful production of cold antihydrogen; the first realization of Bose-Einstein condensation in cesium and ytterbium; the behavior of ultracold bosons and fermions in optical lattices; the production of ultra-cold cesium, helium and fermionic lithium molecules; the production and coherent transport of ultra-cold atoms in microtraps on the surface of chips; the implementation of one-and two-quibit quantum algorithms and experiments towards a scalable quantum computer based on trapped ions; and new medical applications of laser spectroscopy.