Justine Mondry

Lateral organization of cell-matrix proteins on the nanoscale

• Broschiertes Buch

Produktbeschreibung

Cellular anchors are large accumulations of a multitude of multi-functional proteins and are known as focal adhesion sites. In this work, the spatial organization of the cell-matrix proteins Beta3-integrin, Talin, Kindlin 1&2, FAK, Paxillin, Vinculin, Zyxin, Alpha-actinin and Actin was analyzed, using the super-resolution microscopy technique PALM. It was demonstrated, that all cell-matrix proteins form distinct areas of varying densities inside single focal adhesions. In order to study the temporal alterations and formation of highly dense protein accumulations, the dynamic behavior of the adhesion receptor Beta3-integrin was analyzed. It was shown, that force inhibition can induce structural rearrangements, also leading to the redistribution of the density inside adhesion sites. Dense domains could even have a particular signaling function, as it was demonstrated that the signaling protein FAK is primarily recruited to delimited areas inside focal adhesions, which could represent dense domains.

Produktdetails

• Verlag: Südwestdeutscher Verlag für Hochschulschriften
• Seitenzahl: 148
• Erscheinungstermin: 11. Februar 2016
• Englisch
• Abmessung: 220mm x 150mm x 10mm
• Gewicht: 238g
• ISBN-13: 9783838136738
• ISBN-10: 383813673X
• Artikelnr.: 41343342

Autorenporträt

After finishing a Master of Science in biochemistry at the Ruhr-University Bochum in 2009, she started as a PhD student at the Max-Planck-Institute of Molecular Physiology in Dortmund. In early 2014 she graduated and continued as a Postdoc. Since July 2014 she works as a coordinator at an organic chocolate farm in Costa Rica.