This timely book provides a comprehensive overview of the use of AFM and related scanning probe microscopies for cell surface analysis, going from the basics to the applications side. It covers all cell types, going from viruses and protoplasts to bacteria and animal cells and to discuss a range of advanced AFM modalities, including high-resolution imaging, nanoindentation measurements, recognition imaging, and single-molecule and single-cell force spectroscopy. The book covers methodologies for preparing and analyzing cells and membranes of all kinds and highlights recent examples to…mehr
This timely book provides a comprehensive overview of the use of AFM and related scanning probe microscopies for cell surface analysis, going from the basics to the applications side. It covers all cell types, going from viruses and protoplasts to bacteria and animal cells and to discuss a range of advanced AFM modalities, including high-resolution imaging, nanoindentation measurements, recognition imaging, and single-molecule and single-cell force spectroscopy. The book covers methodologies for preparing and analyzing cells and membranes of all kinds and highlights recent examples to illustrate the power of AFM techniques in life sciences and nanomedicine.
Yves Dufrêne received his engineering degree in chemistry and bioindustries (in 1991) and a PhD degree in biophysical chemistry (in 1996) at the Université catholique de Louvain (UCL, Belgium). After a postdoc at the Naval Research Laboratory (Washington DC, USA), he became research associate (2000) of the Belgian National Fund for Scientific Research and lecturer in physical chemistry and nanobiotechnology at UCL. He is currently the head of the Laboratory of Chemistry of Interfaces.
Inhaltsangabe
Observing the Nanoscale Organization of Model Biological Membranes by Atomic Force Microscopy. High-Resolution Atomic Force Microscopy of Native Membranes. Microbial Cell Imaging Using Atomic Force Microscopy. Resolving the High-Resolution Architecture, Assembly and Functional Repertoire of Bacterial Systems by Atomic Force Microscopy. Understanding Cell Secretion and Membrane Fusion Processes on the Nanoscale Using the Atomic Force Microscope. Nanophysiology of Cells, Channels and Nuclear Pores. Topography and Recognition Imaging of Cells. High-Speed Atomic Force Microscopy for Dynamic Biological Imaging. Near-Field Scanning Optical Microscopy of Biological Membranes. Quantifying Cell Adhesion Using Single-Cell Force Spectroscopy. Probing Cellular Adhesion at the Single-Molecule Level. Mapping Membrane Proteins on Living Cells Using the Atomic Force Microscope. Probing Bacterial Adhesion Using Force Spectroscopy. Force Spectroscopy of Mineral-Microbe Bonds. Single-Molecule Force Spectroscopy of Microbial Cell Envelope Proteins. Probing the Nanomechanical Properties of Viruses, Cells and Cellular Structures. Label-Free Monitoring of Cell Signalling Processes Through AFM-Based Force Measurements. Investigating Mammalian Cell Nanomechanics with Simultaneous Optical and Atomic Force Microscopy. The Role of Atomic Force Microscopy in Advancing Diatom Research into the Nanotechnology Era. Atomic Force Microscopy for Medicine.
Observing the Nanoscale Organization of Model Biological Membranes by Atomic Force Microscopy. High-Resolution Atomic Force Microscopy of Native Membranes. Microbial Cell Imaging Using Atomic Force Microscopy. Resolving the High-Resolution Architecture, Assembly and Functional Repertoire of Bacterial Systems by Atomic Force Microscopy. Understanding Cell Secretion and Membrane Fusion Processes on the Nanoscale Using the Atomic Force Microscope. Nanophysiology of Cells, Channels and Nuclear Pores. Topography and Recognition Imaging of Cells. High-Speed Atomic Force Microscopy for Dynamic Biological Imaging. Near-Field Scanning Optical Microscopy of Biological Membranes. Quantifying Cell Adhesion Using Single-Cell Force Spectroscopy. Probing Cellular Adhesion at the Single-Molecule Level. Mapping Membrane Proteins on Living Cells Using the Atomic Force Microscope. Probing Bacterial Adhesion Using Force Spectroscopy. Force Spectroscopy of Mineral-Microbe Bonds. Single-Molecule Force Spectroscopy of Microbial Cell Envelope Proteins. Probing the Nanomechanical Properties of Viruses, Cells and Cellular Structures. Label-Free Monitoring of Cell Signalling Processes Through AFM-Based Force Measurements. Investigating Mammalian Cell Nanomechanics with Simultaneous Optical and Atomic Force Microscopy. The Role of Atomic Force Microscopy in Advancing Diatom Research into the Nanotechnology Era. Atomic Force Microscopy for Medicine.
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