F W Hemming, J N Hawthorne

Lipid Analysis

• Broschiertes Buch

Produktbeschreibung

There is increasing recognition of the diversity and biological importance of lipids. Lipid modifications of other biological molecules are now also the subject of intense research activity. This 'user-friendly' introduction describes the techniques curre

Produktdetails

• Verlag: Taylor & Francis Ltd (Sales)
• Seitenzahl: 192
• Erscheinungstermin: 15. Juni 1996
• Englisch
• Abmessung: 233mm x 154mm x 17mm
• Gewicht: 283g
• ISBN-13: 9781872748986
• ISBN-10: 1872748988
• Artikelnr.: 23515492

Autorenporträt

Department of Biochemistry, Medical School, Queen's Medical Centre, Nottingham NG7 2UH, UK. Department of Biochemistry, Medical School, Queen's Medical Centre, Nottingham NG7 2UH, UK.

Inhaltsangabe

Abbreviations
Preface
1. Introduction
2. Basic Techniques
Extraction methods
Lipid binding in tissues
Solvents for lipid extraction
Some precautions
Storage of lipid extracts
Saponification
Chromatographic procedures
General considerations
Partition chromatography
Adsorption chromatography
Ion exchange chromatography
Complexing chromatography
Spectrometric methods
UV
visible spectrometry
IR spectroscopy
NMR spectroscopy
Mass spectrometry
Radioisotopic methods
Choice of radioisotope
Autoradiography
Scintillation counting
Gas ionization detection
Gamma counting
Radioisotopes in lipid studies
RIA and related techniques
Immunochemical methods
Antibodies to lipids
Hemagglutination in detection and assay
Immunostaining and TLC
Immunoradiometric assays
Enzyme
linked immunosorbent assays
Analytical methods involving enzymes
Phospholipases
Stereospecific analysis of triacylglycerol
Enzymes in quantifying lipids
Presentation of lipids in aqueous systems
Care and safety during the analysis of lipids in biological materials
General considerations
Chemical hazards
Fire hazards
Radioisotope hazards
Biological hazards
Other hazards
Personal preparedness and protection
References
3. Hydrocarbons
Biological significance
Structures
Detection
Isolation and purification
Quantitation
References
4. Alcohols, Phenols, Aldehydes, Ketones and Quinones
Alcohols and phenols
General and biological significance
Structures
Detection
Isolation and purification
Quantitation
Aldehydes and ketones
General and biological significance
Structures
Detection
Isolation and purification
Quantitation
Quinones
General and biological significance
Structures
Detection
Isolation and purification
Quantitation
References
5. Fatty Acids and Prostaglandins
Fatty acids
Types of fatty acids found naturally
Separation methods for fatty acids
GLC of fatty acids
HPLC of fatty acids
Prostaglandins and leukotrienes (eicosanoids)
Analysis of eicosanoids
References
6. Esters
Acylglycerols
Separation of acylglycerols
Analysis of acylglycerols
Fatty acids of acylglycerols
Wax esters
Alkylglycerols
Analytical methods for alkylglycerols
References
7. Phospholipids, Sulfolipids and Related Compounds
Phospholipids with amino
groups
Hydrolytic procedures in analysis
Estimation of phosphate
Separation of phospholipids
Phosphoinositides
Phosphoinositide and inositol phosphate metabolism
Separation of the phosphoinositides
Receptor
linked phosphoinositide changes
Separation and estimation of inositol phosphates
Estimation of diacylglycerol
Phosphatidylinositol glycans
Detection of PI glycans
Phosphonolipids
Separation and analysis of phosphonolipids
Alkylether phospholipids
Analysis of alkylether phospholipids
Sulfolipids
Lipid sulfates
Sulfonolipids
Analytical methods
References
8. Glycolipids
Glycosphingolipids
Biological significance
Structures
Detection
Isolation and purification
Quantitation
Polyisoprenyl phosphosugars
Biological significance
Structures
Detection
Isolation and purification
Quantitation
Glycosylglycerides and related compounds
Biological significance
Structures
Detection
Isolation and purification
Quantitation
Phosphoglycolipids, lipoteichoic acids and related compounds
Biological significance
Structures
Detection, isolation, preparation and quantitation
References
9. Lipoproteins
Plasma lipoproteins
Structures and biological significance
Detection
Isolation and purification
Quantitation
Fatty acyl proteins and prenyl proteins
Biological significance
Structures
Detection
Isolation and purification
Quantitation
References
Appendices
Appendix A: Glossary
Appendix B: Suppliers
Appendix C: Further reading
Index.