Liquid-Liquid Phase Coexistence and Membraneless Organelles

Herausgegeben:Keating, Christine D.

• Gebundenes Buch

Produktbeschreibung

Methods in Enzymology, Volume 646, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this new release include Methods for Studying RNA condensation/granules in vitro, RNA Dynamics in Intracellular Condensates, Methods for Viscoelastic Characterization of Liquid and Gel Condensates, Incorporating Proteins into Complex Coacervates, Methods for Study of Liquid-Liquid Phase Coexistence in Proximity to Lipid Membranes, Preparation of and Solute Partitioning in Multiphase Coacervates, Reversible photocontrol of DNA coacervation, Enzymatic Control over Coacervation, and much more.

Produktdetails

• Methods in Enzymology Volume 646
• Verlag: Academic Press / Elsevier Science & Technology
• Artikelnr. des Verlages: S0076-6879(21)X0002-X
• Englisch
• Abmessung: 22mm x 152mm x 229mm
• Gewicht: 760g
• ISBN-13: 9780128211595
• Artikelnr.: 67579016

Autorenporträt

Christine Keating is at Penn State University, PA, USA

Inhaltsangabe

1. Using a sequence-specific coarse-grained model for studying protein liquid-liquid phase separation



Roshan M. Regy, Wenwei Zheng and Jeetain Mittal



2. Reconstituting bacterial cell division assemblies in crowded, phase-separated media



Begoña Monterroso, Miguel Ángel Robles-Ramos, Silvia Zorrilla and Germán Rivas



3. Terpolymer-stabilized complex coacervates: A robust and versatile synthetic cell platform



Alexander F. Mason, Wiggert J. Altenburg, Shidong Song, Marleen van Stevendaal and Jan C.M. van Hest



4. Cell-free reconstitution of multi-condensate assemblies



Andrea Putnam and Geraldine Seydoux



5. Practical considerations for generation of multi-compartment complex coacervates



Gregory A. Mountain and Christine D. Keating



6. Methods for characterizing the material properties of biomolecular condensates



Ibraheem Alshareedah, Taranpreet Kaur and Priya R. Banerjee



7. Small-angle X-ray scattering experiments of monodisperse intrinsically disordered protein samples close to the solubility limit



Erik W. Martin, Jesse B. Hopkins and Tanja Mittag



8. SAXS methods for investigating macromolecular and self-assembled polyelectrolyte complexes



Amanda B. Marciel, Samanvaya Srivastava, Jeffrey M. Ting and Matthew V. Tirrell



9. Ultra-small angle neutron scattering to study droplet formation in polyelectrolyte complex coacervates



Vivek M. Prabhu, Samim Ali, Markus Bleuel, Yimin Mao and Yuanchi Ma



10. Incorporation of proteins into complex coacervates



Whitney C. Blocher McTigue and Sarah L. Perry



11. Measuring the activity and structure of functional RNAs inside compartments formed by liquid-liquid phase separation



Raghav R. Poudyal, McCauley O. Meyer and Philip C. Bevilacqua



12. Reversible photocontrol of DNA coacervation



Suzanne Lafon and Nicolas Martin



13. Enzymatic control over coacervation



Karina K. Nakashima, Alain A.M. André and Evan Spruijt