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Malaria is a major public health problem in Ethiopia; mainly due to the large Mobile community and climate change. Teltele Woreda is one of the high malaria transmission stratified districts in the land area of Borena Zone. Objective To assess utilisation of treated bed net in malaria prevention among individuals in Teltele district, Borena zone, Ethiopia from July 15-August 31, 2022.Methods: An institution-based age, resident, and gender-matched case-control study was conducted among 300 samples (75 cases and 225 controls). Cases were individuals tested by microscopy and get positive for malaria at health centers and hospitals, controls were matched by age, resident and gender with cases by interval and individual matching respectively. Data were collected using a structured questionnaire, entered into Epi-data version 3.1 and matched cases and controls were paired by grouping with the same codes. The data were exported to STATA version 14.0 for analysis. Results: About 29(39%) cases and 130(58.5%) controls slept under bed net regularly for two weeks before data collection. The study shows odds of malaria infection are reduced by 71.6% among individuals who use bed nets regularly.