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During laparoscopic surgery, insufflation of carbon dioxide for the creation of Pneumoperitoneum (PP) causes multiple hemodynamic variations. The aim of our study is to investigate the efficacy of magnesium sulfate in preventing adverse hemodynamic reactions associated with PP in patients undergoing laparoscopic cholecystectomy.Methods: This was a prospective, randomized, double-blind clinical trial conducted at the Department of Anesthesia and Intensive Care, CHU Habib Bourguiba Sfax. The study included seventy patients aged between 20 and 65 undergoing laparoscopic cholecystectomy, who were randomized into two groups containing 35 patients each.The Mg2+ group: received 50 mg/kg intravenous magnesium sulfate diluted in 100 ml saline prior to anesthetic induction.Group S: received the same volume of 0.9% saline before anesthetic induction.Results and conclusion: Magnesium sulfate administered prior to PP insufflation attenuated the unfavorable hemodynamic response and provided hemodynamic stability.