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The family of extracellular ligninolytic enzymes typically includes lignin peroxidases laccases, manganese peroxidases, versatile peroxidases and other accessory enzymes. Out of these enzymes MnP is thought to play the most crucial role in lignin degradation. Manganese peroxidase, is an ubiquitous enzyme among ligninolytic fungi, is an extracellular heme- containing enzyme known to catalyse the oxidation of Mn2+ to Mn3+ in a reaction requiring appropriate manganese chelator. This enzyme catalysis the oxidation of Mn2+ to Mn3+, which in turn can oxidize a variety of phenolic substrates, including lignin model compounds 3 fungi were isolated from soil samples from different regions in Bangalore. Isolated fungal strains were screened for Manganese peroxidase production by plate assay. Aspergillus sps VV01 were selected as best Manganese peroxidase producer.