Manual of Molecular and Clinical Laboratory Immunology

Herausgegeben:Detrick, Barbara; Hamilton, Robert G.; Schmitz, John

• Gebundenes Buch

Produktbeschreibung

THE authoritative guide for clinical laboratory immunology

For over 40 years the Manual of Molecular and Clinical Laboratory Immunology has served as the premier guide for the clinical immunology laboratory. From basic serology testing to the present wide range of molecular analyses, the Manual has reflected the exponential growth in the field of immunology over the past decades. This eighth edition reflects the latest advances and developments in the diagnosis and treatment of patients with infectious and immune-mediated disorders. The Manual features detailed descriptions of general and specific methodologies, placing special focus on the interpretation of laboratory findings, and covers the immunology of infectious diseases, including specific pathogens, as well as the full range of autoimmune and immunodeficiency diseases, cancer, and transplantation.

Written to guide the laboratory director, the Manual will also appeal to other laboratory scientists, especially those working in clinical immunology laboratories, and pathologists. It is also a useful reference for physicians, mid-level providers, medical students, and allied health students with an interest in the role that immunology plays in the clinical laboratory.

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Produktdetails

• ASM
• Verlag: Wiley / Wiley & Sons
• Artikelnr. des Verlages: 1W555818710
• 8. Aufl.
• Seitenzahl: 1240
• Erscheinungstermin: 15. Februar 2016
• Englisch
• Abmessung: 284mm x 215mm x 53mm
• Gewicht: 666g
• ISBN-13: 9781555818715
• ISBN-10: 1555818714
• Artikelnr.: 42801126

Inhaltsangabe

Editorial Board xi

Contributors xiii

Foreword: How it Began xxiii

Preface xxv

Author and Editor Conflicts of Interest xxvii

Section A General Methods 1

Volume Editor: Robert G. Hamilton

Section Editor: Thomas A. Fleisher

1 Introduction 3
Thomas A. Fleisher

2 Molecular Methods for Diagnosis of Genetic Diseases Involving the Immune System 5
Amy P. Hsu

3 The Human Microbiome and Clinical Immunology 19
Frederic D. Bushman

4 Protein Analysis in the Clinical Immunology Laboratory 26
Roshini Sarah Abraham and David R. Barnidge

Section B Immunoglobulin Methods =/ 47

Volume Editor: Robert G. Hamilton

Section Editor: David F. Keren

5 Introduction 49
David F. Keren

6 Immunoglobulin Genes 51
Thomas J. Kipps, Emanuela M. Ghia, and Laura Z. Rassenti

7 Immunoglobulin Quantification and Viscosity Measurement 65
Jeffrey S. Warren

8 Clinical Indications and Applications of Serum and Urine Protein Electrophoresis 74
David F. Keren and Richard L. Humphrey

9 Immunochemical Characterization of Immunoglobulins in Serum, Urine, and Cerebrospinal Fluid 89
Elizabeth Sykes and Yvonne Posey

10 Cryoglobulins, Cryofibrinogenemia, and Pyroglobulins 101
Peter D. Gorevic and Dennis Galanakis

11 Strategy for Detecting and Following Monoclonal Gammopathies 112
Jerry A. Katzmann and David F. Keren

Section C Complement 125

Volume Editor: Robert G. Hamilton

Section Editor: Patricia C. Giclas

12 Introduction 127
Patricia C. Giclas

13 The Classical Pathway of Complement 129
Patricia C. Giclas

14 Analysis of Activity of Mannan-Binding Lectin, an Initiator of the Lectin Pathway of the Complement System 133
Steffen Thiel

15 The Nature of the Diseases That Arise from Improper Regulation of the Alternative Pathway of Complement 138
Richard J. H. Smith

Section D Flow Cytometry 145

Volume Editor: John L. Schmitz

Section Editor: Maurice R. G. O'gorman

16 Introduction 147
Maurice R. G. O'gorman

17 Polychromatic Flow Cytometry 149
Angélique Biancotto and J. Philip Mccoy, Jr.

18 High-Sensitivity Detection of Red and White Blood Cells in Paroxysmal Nocturnal Hemoglobinuria by Multiparameter Flow Cytometry 168
Andrea Illingworth, Michael Keeney, and D. Robert Sutherland

19 Standardized Flow Cytometry Assays for Enumerating CD34+ Hematopoietic Stem Cells 182
D. Robert Sutherland and Michael Keeney

20 Functional Flow Cytometry-Based Assays of Myeloid and Lymphoid Functions for the Diagnostic Screening of Primary Immunodeficiency Diseases 199
Maurice R. G. O'gorman

21 Acute Lymphoblastic Leukemia/Lymphoma: Diagnosis and Minimal Residual Disease Detection by Flow Cytometric Immunophenotyping 207
Joseph A. Digiuseppe

22 Acute Myeloid Leukemia: Diagnosis and Minimal Residual Disease Detection by Flow Cytometry 217
Brent Wood and Lori Soma

23 Chronic Lymphocytic Leukemia, the Prototypic Chronic Leukemia for Flow Cytometric Analysis 226
Heba Degheidy, Dalia A. A. Salem, Constance M. Yuan, and Maryalice Stetler-Stevenson

24 Plasma Cell Disorders 235
Juan Flores-Montero, Luzalba Sanoja, José Juan Pérez, Fanny Pojero, Noemí Puig, María Belén Vidriales, and Alberto Orfao

25 Future Cytometric Technologies and Applications 251
Holden T. Maecker

Section E Functional Cellular Assays 259

Volume Editor: Barbara Detrick

Section Editor: Steven D. Douglas

26 Introduction 261
Steven D. Douglas

27 Cryopreservation of Peripheral Blood Mono