Manual of Research Techniques in Cardiovascular Medicine

Herausgegeben von Ardehali, Hossein; Bolli, Roberto; Losordo, Douglas W.

• Gebundenes Buch

Produktbeschreibung

While some research methods or techniques are applicable in severalareas of medicine, research in cardiovascular diseases requiresknowledge of an increasing array of procedures, techniques andmeasurements that are highly specialized and unique to this area ofinvestigation. Edited by senior clinical investigators who arerecognized leaders in cardiovascular medicine worldwide, this bookprovides readers with a comprehensive, practical"how-to-do-it" review of best-practice techniques forcardiovascular research.

Produktdetails

• Verlag: Wiley & Sons
• 1. Auflage
• Seitenzahl: 472
• Erscheinungstermin: 17. März 2014
• Englisch
• Abmessung: 257mm x 183mm x 25mm
• Gewicht: 1134g
• ISBN-13: 9780470672693
• ISBN-10: 0470672692
• Artikelnr.: 40133785

Autorenporträt

Edited by Hossein Ardehali MD, PhD, FAHA, Associate Professor of Medicine, Molecular Pharmacology and Biological Chemistry Feinberg Cardiovascular Research Institute Northwestern University Feinberg School of Medicine, Chicago, IL USA Roberto Bolli MD Professor of Medicine Chief, Division of Cardiovascular Medicine Director, Institute of Molecular Cardiology University of Louisville Louisville, KY USA Douglas W. Losordo MD Vice President New Therapeutic Development Baxter Healthcare Corporation Deerfield, IL USA

Inhaltsangabe

List of Contributors ix Preface xix About the Companion Website xx Part 1
Electrophysiology 1 Measurement of calcium transient ex vivo 3 Kenneth R.
Laurita and Bradley N. Plummer 2 Confocal imaging of intracellular calcium
cycling in isolated cardiac myocytes 12 Søren Grubb, J. Andrew Wasserstrom,
and Gary L. Aistrup 3 Generating a large animal model of persistent atrial
fi brillation 20 Raphaël P. Martins and José Jalife 4 Confocal imaging of
intracellular calcium cycling in the intact heart 32 Neha Singh, Manvinder
Kumar, James E. Kelly, Gary L. Aistrup, and J. Andrew Wasserstrom 5
Recording and measurement of action potentials 41 Kenneth S. Ginsburg and
Donald M. Bers 6 Patch-clamp recordings from isolated cardiac myocytes 50
Kai-Chien Yang, Wei Wang, and Jeanne M. Nerbonne 7 Optical mapping of the
heart 60 Di Lang, Sarah Gutbrod, Jacob Laughner, and Igor R. Efi mov Part 2
Isolation and Maintenance of Primary Stem Cells 8 Isolation of
colony-forming endothelial progenitor cells 71 Haruchika Masuda and
Takayuki Asahara 9 Cardiac resident stem cells 87 João Ferreira-Martins,
Fumihiro Sanada, and Marcello Rota 10 Cardiospheres 95 Rachel Ruckdeschel
Smith 11 Mesenchymal stem cells 104 Jose S. Da Silva and Joshua M. Hare 12
Generation and differentiation of human iPS cells 110 Sebastian Diecke, Lei
Ye, Sophia Zhang, and Jianyi Zhang 13 Isolation of neonatal and adult rat
cardiomyocytes 117 Md. Abdur Razzaque and Jeffrey Robbins 14 Isolation and
culture of vascular smooth muscle cells 125 Milton Hamblin, Lin Chang, and
Y. Eugene Chen 15 Isolation and culture of cardiac endothelial cells 131
Asish K. Ghosh, Joseph W. Covington, and Douglas E. Vaughan 16 Isolation
and culture of cardiac fi broblasts 140 Asish K. Ghosh, Joseph W. Covington
and Douglas E. Vaughan 17 Murine bone marrow transplantation model 146
Prasanna Krishnamurthy, Suresh Kumar Verma, and Raj Kishore 18 In vitro
differentiation and expansion of vascular endothelial cells derived from
mouse embryonic stem cells 149 Anees Fatima, Carey Nassano-Miller, and
Tsutomu Kume Part 3 Manipulation of the Heart and Vessels in Vivo and ex
Vivo 19 Coronary ligation 157 Alexander R. Mackie and Hossein Ardehali 20
Transverse aortic constriction: a model to study heart failure in small
animals 164 Suresh Kumar Verma, Prasanna Krishnamurthy, and Raj Kishore 21
Pharmacological models of hypertrophy and failure 170 Angela C. deAlmeida,
Tariq Hamid, and Sumanth D. Prabhu 22 Hindlimb ischemia 177 Jerry C. Lee,
Ngan F. Huang, and John P. Cooke 23 The Langendorff preparation 187 Hugh
Clements-Jewery and Michael J. Curtis 24 Myocarditis and other
immunological models of cardiac disease 197 Daniela C¡ iháková and Noel R.
Rose 25 Models of pacing-induced heart failure 203 James A. Shuman, Rupak
Mukherjee, and Francis G. Spinale 26 Porcine myocardial ischemia models 215
Xian-Liang Tang and Roberto Bolli 27 Angiogenesis assays 225 Susmita Sahoo
and Douglas W. Losordo 28 Immunohistochemical analysis of cardiac tissue
232 Barbara Ogórek, Donato Cappetta, and Jan Kajstura 29 A murine model of
cardiac arrest by exsanguination 237 Guangming Cheng, Yiru Guo, Harold K.
Elias, Carrie M. Quinn, Arash Davani, Yanjuan Yang, Magdy Girgis, Roberto
Bolli, and Buddhadeb Dawn Part 4 Small Animal Imaging 30 Blood pressure,
telemetry, and vascular measurements in the rodent model 245 Robert S.
Danziger 31 The setting: imaging conscious, sedated, or anesthetized
rodents 250 Gene H. Kim and Roberto M. Lang 32 Echocardiography: standard
techniques (M-mode, two-dimensional imaging, and Doppler) 254 Gene H. Kim,
Lauren Beussink-Nelson, Sanjiv J. Shah, and Roberto M. Lang 33
Echocardiography: advanced techniques (tissue Doppler, speckle tracking,
and three-dimensional imaging) 275 Lauren Beussink-Nelson, Gene H. Kim,
Roberto M. Lang, and Sanjiv J. Shah 34 In vivo tomographic cardiac imaging:
positron emission tomography and magnetic resonance imaging 287 Bruno C.
Huber, Patricia K. Nguyen, and Joseph C. Wu 35 In vivo hemodynamics 295
Alexander R. Mackie, Kyle K. Henderson, Sol Misener, and Hossein Ardehali
Part 5 Metabolism, Mitochondria, and Cell Death 36 Fractionation of
cardiomyocytes and isolation of mitochondria 307 Christopher P. Baines 37
Assessment of glucose and fatty acid metabolism ex vivo 313 Darrell D.
Belke and E. Dale Abel 38 Quantification and characterization of
atherosclerotic lesions in mice 320 Abhinav Agarwal, Millicent G. Winner,
Srinivas D. Sithu, and Sanjay Srivastava 39 Assessment of cell death in the
heart 332 Russell S. Whelan, Klitos Konstantinidis, and Richard N. Kitsis
40 Assessment of mitochondrial function in isolated cells 343 Amy K. Rines
and Hossein Ardehali 41 Multinuclear NMR spectroscopy of myocardial
energetics and substrate utilization in isolated perfused mouse hearts 351
Stephen C. Kolwicz, Jr. and Rong Tian 42 Measurement of reactive oxygen
species in cardiovascular disease 359 Mahmood Khan, Fatemat Hassan,
Sashwati Roy, and Chandan K. Sen 43 Assessing autophagy 371 Roberta A.
Gottlieb 44 Assessment of cardiomyocyte size 378 A. Martin Gerdes and
Alessandro Pingitore Part 6 Manipulation of Gene Expression in Vitro and in
Vivo 45 Generation of Cre-loxP mouse models for conditional knockout and
overexpression of genes in various heart cells 389 Marisa Z. Jackson and
Warren G. Tourtellotte 46 Modulation of myocardial genes via use of
adenoviral vectors and RNA interference approaches 397 Qianhong Li 47
Overexpression and downregulation of proteins in vitro 407 Marina Bayeva
and Hossein Ardehali 48 In vivo microRNA studies 416 Eva van Rooij Part 7
Model Systems 49 Vascular and cardiac studies in zebrafi sh 425 Hans-Georg
Simon, Molly Ahrens, and Brandon Holtrup 50 Vascular and cardiac studies in
Drosophila 432 Lin Yu, Joseph P. Daniels, and Matthew J. Wolf Index 441