Traditional knowledge is employed to mean knowledge, innovations and practices of indigenous and local communities embodying traditional life-styles; the wisdom developed over many generations of holistic traditional scientific utilization of the lands, natural resources, and environment. Traditional knowledge is valid and necessary, and awaits its currently relevant wider application for human benefit. Many people in Mediterranean region who consult with spiritual healers, homeopaths and herbalists are utilizing traditional therapies. These are the first choice for problems such as liver diseases, inflammation, skin diseases, infertility, impotence, diabetes, obesity, epilepsy, psychosomatic troubles, and many other diseases. In this book author present some of the most important issues for medical plants in rural regions of Iran and their Traditional knowledge and economic importance for Saffron, Ferula, Jujube, Astragalus and Barberry. Keywords: Traditional knowledge, rural, Saffron, Ferula, Jujube, Astragalus, Barberry, Iran.