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By using microbial cell factories for the production of aromatics from sustainable substrates, drawbacks such as dependency on fossil resources as well as unfavorable production conditions can be circumvented.This dissertation deals with the rational and non-rational metabolic engineering of Pseudomonas based biocatalysts for the production of 4-hydroxybenzoate in high yields from glucose and glycerol. The gained results bring the bio-economic formation of aromatics one step closer.