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Two different Schiff bases were prepared by condensation between isatin drug and diaminopyridine or isoleucine amino acid and characterized using different physio-chemical techniques. The prepared ligands were used for synthesizing different transition metal complexes. The prepared metal complexes were identified using different tools like FT-IR, Mass, SEM, XRD, elemental analysis , 1HNMR, etc. Also screening its aantimicrobial activity and anticancer activity were done. The data confirmed the high activity of the ligands and their chelates.