Methods for Analysis of Golgi Complex Function

Volume 118

• Gebundenes Buch

Produktbeschreibung

This new volume of Methods in Cell Biology looks at methods for analyzing of golgi complex function. Chapters cover such topics as in vitro reconstitution systems, fluorescence-based analysis of trafficking in mammalian cells and high content screening. With cutting-edge material, this comprehensive collection is intended to guide researchers for years to come.

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Produktdetails

• Verlag: Elsevier Science
• Seitenzahl: 498
• Erscheinungstermin: 21. November 2013
• Englisch
• Abmessung: 241mm x 192mm x 30mm
• Gewicht: 1163g
• ISBN-13: 9780124171640
• ISBN-10: 0124171648
• Artikelnr.: 39171660

Inhaltsangabe

Model systems and functional studies1.Analysis of Golgi Complex Functions:
In vitro Reconstitution Systems2.RNA Interference Approaches to Examine
Golgi Function in Animal Cell Culture3.Trafficking along the Secretory
Pathway in Drosophila Cell Line and Tissues: A Light and Electron
Microscopy Approach4.Studying Membrane Trafficking in the Worm C. elegans
by RNA Interference5.Endomembrane and Golgi Traffic in Plant
Cells6.Biochemical Analysis of Secretory Trafficking in Mammalian Cells
7.Methods for Analysis of Apical Lumen Trafficking Using Micropatterned 3D
Systems8.Using Replication Defective Viruses to Analyze Membrane
Trafficking in Polarized Epithelial Cells9.Snap-Tagging the Retrograde
Route10.Assessing ER and Golgi N-Glycosylation Process using Metabolic
Labeling in Mammalian Cultured Cells
Imaging based approaches11.Fluorescence based Analysis of Trafficking in
Mammalian Cells12.Photobleaching Methods to study Golgi Complex Dynamics in
Living Cells13.Photohighlighting approaches to access membrane dynamics of
the Golgi apparatus14.Live Cell Visualization of Golgi Membrane Dynamics by
Super-Resolution Confocal Live Imaging Microscopy (SCLIM)15.Analysis of
Golgi Complex Function using Correlative Light-Electron Microscopy
(CLEM)16.Viewing Golgi Structure and Function from a different perspective
- Insights from Electron Tomography17.High Content Screening and Analysis
of the Golgi Complex
Emerging studies [New Frontiers]18.(Re)Modeling the Golgi19.Golgi Depletion
from Living Cells with Laser Nanosurgery20.Studying Intracellular
Trafficking Pathways with Probabilistic Density Maps21.Methods to Study
Signaling at The Golgi Apparatus22.Golgi-Dependent Signaling:
Self-Coordination of Membrane Trafficking23.Signaling at the Golgi during
Mitosis24.Ice Recovery Assay for Detection of Golgi-Derived Microtubules