Table of contents:
Approaches to Novel Research
Rapid Isolation of Specific cDNAs or Genes by PCR
Construction and Screening of Subtracted and Complete Expression cDNA Libraries
Subcloning of Genes or DNA Fragments
Nonisotopic and Isotopic DNA or RNA Sequencing
Information Superhighway and Computer Databases of Nucleic Acids and Proteins
Characterization of DNA or Genes by Southern Blot Hybridization
Gene Overexpression by Sense RNA in Mammalian Systems
Gene Underexpression in Cultured Cells and Animals by Antisense DNA and RNA Strategies
Analysis of Gene Expression at the RNA Level
Analysis of Gene Expression at the Protein Level
Analysis of Cellular DNA or Abundance mRNA by Radioactive In Situ Hybridization (RISH)
Localization of DNA or Abundance of mRNA by Fluorescence In Situ Hybridization (FISH)
In Situ PCR Hybridization of Low Copy Genes and In Situ RT-PCR Detection of Low Abundance mRNAs
Isolation and Characterization of Genes from Genomic DNA Libraries
Culture of Mouse Embry onic Stem Cells as a Model Mammalian Cell Line for Gene Expression
New Strategies for Gene Knockout
Large Scale Expression and Purification of Recombinant Proteins in Cultured Cells
Index
All chapters contain Troubleshooting Guides and References
Many scientists find themselves working with genes in the laboratory without sufficient background in current biotechnology methods. Others want to keep up with the revolution in biotechnology and the associated flood of new methodologies. Methods in Gene Biotechnology provides the complete, broad-based coverage that researchers and students require to understand and apply today's biotechnology techniques. It discusses a wide variety of approaches, from very basic methods to the latest, most sophisticated technologies.
Written by a team of internationally recognized scientists and scholars, Methods in Gene Biotechnology offers several notable features typically lacking in other works on the subject. The book presents protocol recipes, and also provides clear and simple explanations of the key principles and concepts behind the methods. It includes useful troubleshooting guides to help solve experimental problems, presenting a single, logically organized source for all the most important new methodologies. This unique resource provides the tools for success in molecular and cellular biology research today.
Approaches to Novel Research
Rapid Isolation of Specific cDNAs or Genes by PCR
Construction and Screening of Subtracted and Complete Expression cDNA Libraries
Subcloning of Genes or DNA Fragments
Nonisotopic and Isotopic DNA or RNA Sequencing
Information Superhighway and Computer Databases of Nucleic Acids and Proteins
Characterization of DNA or Genes by Southern Blot Hybridization
Gene Overexpression by Sense RNA in Mammalian Systems
Gene Underexpression in Cultured Cells and Animals by Antisense DNA and RNA Strategies
Analysis of Gene Expression at the RNA Level
Analysis of Gene Expression at the Protein Level
Analysis of Cellular DNA or Abundance mRNA by Radioactive In Situ Hybridization (RISH)
Localization of DNA or Abundance of mRNA by Fluorescence In Situ Hybridization (FISH)
In Situ PCR Hybridization of Low Copy Genes and In Situ RT-PCR Detection of Low Abundance mRNAs
Isolation and Characterization of Genes from Genomic DNA Libraries
Culture of Mouse Embry onic Stem Cells as a Model Mammalian Cell Line for Gene Expression
New Strategies for Gene Knockout
Large Scale Expression and Purification of Recombinant Proteins in Cultured Cells
Index
All chapters contain Troubleshooting Guides and References
Many scientists find themselves working with genes in the laboratory without sufficient background in current biotechnology methods. Others want to keep up with the revolution in biotechnology and the associated flood of new methodologies. Methods in Gene Biotechnology provides the complete, broad-based coverage that researchers and students require to understand and apply today's biotechnology techniques. It discusses a wide variety of approaches, from very basic methods to the latest, most sophisticated technologies.
Written by a team of internationally recognized scientists and scholars, Methods in Gene Biotechnology offers several notable features typically lacking in other works on the subject. The book presents protocol recipes, and also provides clear and simple explanations of the key principles and concepts behind the methods. It includes useful troubleshooting guides to help solve experimental problems, presenting a single, logically organized source for all the most important new methodologies. This unique resource provides the tools for success in molecular and cellular biology research today.