This well-organized text gives a concise account of various enzymes like protease & keratinase & aims to put the current knowledge into perspective. Proteases are a unique class of enzymes, since they have of immense physiological & commercial importance. They occur ubiquitously in animals, plants & microbes. However, microbes are a goldmine of proteases & represent the preferred source of enzymes by virtue of their rapid growth, limited space required for cultivation & ready accessibility to genetic manipulation. Microbial proteases have been extensively used in the food, dairy & detergent industries since ancient times. The development of recombinant rennin & its commercialization is an excellent example of the successful application of modern biology to biotech. This text discusses the behavior of isolated enzymes- protease & keratinase, dealing in turn with isolation, characterization & purification methods from isolated micro-organisms of soil sample of poultry waste site & various potential industrial applications of enzymes. These methods for isolation, characterization & purification of enzymes are necessary to know for its commercialization at large scale in industry.