In this paper the microbiological method of action of various microorganisms on the quality of the soil will be shown, as well as the monitoring of soil quality through chemical indicators and parameters. Parallels were made in the testing of soil quality after floods, which occurred just in the period when fruit trees and cereals were in development. The sites with which the soil samples were taken are in the valley of the South Morava River on the territory of the Pcinja District. By utilizing appropriate quality parameters, it was concluded that quality should be the land on which the appropriate crops will be planted. Different results of pH, humus, nitrogen, phosphorus and potassium, are obtaindin the function of time, within the research conducted. Experiment was implemented two years ago, immediately after the flood and six months after the floods, which occurred in May 2014 in the region South Serbia. The methods that were used for the analysis of the soil are: colorimetric, photometric, potentiometric and spectrophotometri. Results show a high degree of sustainability of quality of land thet relatively quickly regenerate their properties after floods.