This book aims to provide the hands on training in Microbial analysis. Microbiology concepts and protocols viz.microscopy, microbial culture media, isolation and staining techniques like Gram's staining, endospore staining, negative staining, hanging drop technique to know mobility of microbes and some experiments on environmental factors affecting microbial growth. This book will helps to graduate and post graduate students to operate precise procedure in simple and illustrative format. Researchers can follow this book as handbook while doing microbial analysis.