Microtubules: In Vivo
Volume 97
Microtubules: In Vivo
Volume 97
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Microtubules in Cells is complemented by the companion volume by Correia that covers methods to study microtubules in vitro. The Microtubules in Cells volume includes chapters by experts around the world on many aspects of microtubule imaging in living and fixed cells; assays to study microtubule function in a wide array of model organisms and cultured cells; and high-resolution approaches to study the cytoskeleton. The authors share their years of experience, outlining potential pitfalls and critical factors to consider in experimental design, experimental implementation and data interpretation.…mehr
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Microtubules in Cells is complemented by the companion volume by Correia that covers methods to study microtubules in vitro. The Microtubules in Cells volume includes chapters by experts around the world on many aspects of microtubule imaging in living and fixed cells; assays to study microtubule function in a wide array of model organisms and cultured cells; and high-resolution approaches to study the cytoskeleton. The authors share their years of experience, outlining potential pitfalls and critical factors to consider in experimental design, experimental implementation and data interpretation.
Produktdetails
- Produktdetails
- Verlag: Elsevier Health Sciences
- Seitenzahl: 552
- Erscheinungstermin: 26. August 2010
- Englisch
- Abmessung: 242mm x 199mm x 25mm
- Gewicht: 1050g
- ISBN-13: 9780123813497
- ISBN-10: 0123813492
- Artikelnr.: 30361739
- Verlag: Elsevier Health Sciences
- Seitenzahl: 552
- Erscheinungstermin: 26. August 2010
- Englisch
- Abmessung: 242mm x 199mm x 25mm
- Gewicht: 1050g
- ISBN-13: 9780123813497
- ISBN-10: 0123813492
- Artikelnr.: 30361739
1. Microtubule dynamics at the cell cortex probed by TIRF microscopy
Ilya Grigoriev and Anna Akhmanova
2. Microscopy methods for the study of centriole biogenesis and function
in Drosophila
Ana Rodrigues Martins, Pedro Machado, Giuliano Callaini and Monica
Bettencourt-Dias
3. Quality control in single molecule studies of kinesins and
microtubule-associated proteins
Gary J. Brouhard
4. Imaging cilia in zebrafish
Kimberly M. Jaffe, Stephan Y. Thiberge, Margaret E. Bisher and
Rebecca D. Burdine
5. Automatic Identification of Microtubules in Cellular Electron
Tomography
Daniyar Nurgaliev, Timur Gatanov and Daniel J. Needleman
6. Imaging and analysis of the microtubule cytoskeleton in Giardia
Scott C. Dawson and Susan A. House
7. Kinetochore-microtubule dynamics and attachment stability
Jennifer G. DeLuca
8. Assessment of mitotic spindle phenotypes in Drosophila S2 cells
Gohta Goshima
9. Live cell imaging techniques for analyses of microtubules in
Dictyostelium
Matthias Samereier, Irene Meyer, Michael P. Koonce and Ralph Gräf
10. Microtubule dynamics in dendritic spines
Lukas C. Kapitein , Kah Wai Yau and Casper C. Hoogenraad
11. Analysis of Microtubules in Budding Yeast
Alexander Rauch, Elena Nazarova and Jackie Vogel
12. Determination of Microtubule Dynamic Instability in Living Cells
Kathy Kamath, Emin Oroudjev, and Mary Ann Jordan
13. Modeling microtubule-mediated forces and centrosome positioning in
Caenorhabditis elegans embryos
Akatsuki Kimura1 and Shuichi Onami
14. Cryo electron tomography of cellular microtubules
Roman I. Koning
15. Microtubule dynamics in plant cells
Henrik Buschmann, Adrian Sambade, Edouard Pesquet, Grant Calder and
Clive W. Lloyd
16. Drosophila S2 cells as a model system to investigate mitotic spindle
dynamics, architecture and function
Sara Moutinho-Pereira, Irina Matos and Helder Maiato
17. Protein micropatterns: a direct printing protocol using deep UVs
Ammar Azioune, Nicolas Carpi, Manuel Théry and Matthieu Piel
18. Optical trapping and laser ablation of microtubules in fission yeast
Nicola Maghelli and Iva M. Tolic-Nørrelykke
19. Melanophores for Microtubule dynamics and motility assays
Kazuho Ikeda, Irina Semenova, Olga Zhapparova, and Vladimir Rodionov
20. New and old reagents for fluorescent-protein tagging of microtubules
in fission yeast: experimental and critical evaluation
Hilary A. Snaith, Andreas Anders, Itaru Samejima and Kenneth E. Sawin
21. Imaging of mitotic spindle dynamics in C. elegans embryos
Mika Toya, Yumi Iida and Asako Sugimoto
22. A fast microfluidic temperature control device for studying
microtubule dynamics in fission yeast
Guilhem Velve-Casquillas, Judite Costa, Frédérique Carlier-Grynkorn ,
Adeline Mayeux and Phong T. Tran
23. Microtubule-dependent spatial organization of mitochondria in fission
yeast
Maitreyi Das, Stephan Chiron and Fulvia Verde
24. Photoactivatable GFP-Tubulin
U. Serdar Tulu*, Nick P. Ferenz
25. The Use of Fluorescence Redistribution After Photobleaching for
Analysis of Cellular Microtubule Dynamics
Claire E. Walczak, Rania S. Rizk and Sidney L. Shaw
26. Analysis of microtubule polymerization dynamics in live cells
Sarah Gierke, Praveen Kumar, and Torsten Wittmann
Ilya Grigoriev and Anna Akhmanova
2. Microscopy methods for the study of centriole biogenesis and function
in Drosophila
Ana Rodrigues Martins, Pedro Machado, Giuliano Callaini and Monica
Bettencourt-Dias
3. Quality control in single molecule studies of kinesins and
microtubule-associated proteins
Gary J. Brouhard
4. Imaging cilia in zebrafish
Kimberly M. Jaffe, Stephan Y. Thiberge, Margaret E. Bisher and
Rebecca D. Burdine
5. Automatic Identification of Microtubules in Cellular Electron
Tomography
Daniyar Nurgaliev, Timur Gatanov and Daniel J. Needleman
6. Imaging and analysis of the microtubule cytoskeleton in Giardia
Scott C. Dawson and Susan A. House
7. Kinetochore-microtubule dynamics and attachment stability
Jennifer G. DeLuca
8. Assessment of mitotic spindle phenotypes in Drosophila S2 cells
Gohta Goshima
9. Live cell imaging techniques for analyses of microtubules in
Dictyostelium
Matthias Samereier, Irene Meyer, Michael P. Koonce and Ralph Gräf
10. Microtubule dynamics in dendritic spines
Lukas C. Kapitein , Kah Wai Yau and Casper C. Hoogenraad
11. Analysis of Microtubules in Budding Yeast
Alexander Rauch, Elena Nazarova and Jackie Vogel
12. Determination of Microtubule Dynamic Instability in Living Cells
Kathy Kamath, Emin Oroudjev, and Mary Ann Jordan
13. Modeling microtubule-mediated forces and centrosome positioning in
Caenorhabditis elegans embryos
Akatsuki Kimura1 and Shuichi Onami
14. Cryo electron tomography of cellular microtubules
Roman I. Koning
15. Microtubule dynamics in plant cells
Henrik Buschmann, Adrian Sambade, Edouard Pesquet, Grant Calder and
Clive W. Lloyd
16. Drosophila S2 cells as a model system to investigate mitotic spindle
dynamics, architecture and function
Sara Moutinho-Pereira, Irina Matos and Helder Maiato
17. Protein micropatterns: a direct printing protocol using deep UVs
Ammar Azioune, Nicolas Carpi, Manuel Théry and Matthieu Piel
18. Optical trapping and laser ablation of microtubules in fission yeast
Nicola Maghelli and Iva M. Tolic-Nørrelykke
19. Melanophores for Microtubule dynamics and motility assays
Kazuho Ikeda, Irina Semenova, Olga Zhapparova, and Vladimir Rodionov
20. New and old reagents for fluorescent-protein tagging of microtubules
in fission yeast: experimental and critical evaluation
Hilary A. Snaith, Andreas Anders, Itaru Samejima and Kenneth E. Sawin
21. Imaging of mitotic spindle dynamics in C. elegans embryos
Mika Toya, Yumi Iida and Asako Sugimoto
22. A fast microfluidic temperature control device for studying
microtubule dynamics in fission yeast
Guilhem Velve-Casquillas, Judite Costa, Frédérique Carlier-Grynkorn ,
Adeline Mayeux and Phong T. Tran
23. Microtubule-dependent spatial organization of mitochondria in fission
yeast
Maitreyi Das, Stephan Chiron and Fulvia Verde
24. Photoactivatable GFP-Tubulin
U. Serdar Tulu*, Nick P. Ferenz
25. The Use of Fluorescence Redistribution After Photobleaching for
Analysis of Cellular Microtubule Dynamics
Claire E. Walczak, Rania S. Rizk and Sidney L. Shaw
26. Analysis of microtubule polymerization dynamics in live cells
Sarah Gierke, Praveen Kumar, and Torsten Wittmann
1. Microtubule dynamics at the cell cortex probed by TIRF microscopy
Ilya Grigoriev and Anna Akhmanova
2. Microscopy methods for the study of centriole biogenesis and function
in Drosophila
Ana Rodrigues Martins, Pedro Machado, Giuliano Callaini and Monica
Bettencourt-Dias
3. Quality control in single molecule studies of kinesins and
microtubule-associated proteins
Gary J. Brouhard
4. Imaging cilia in zebrafish
Kimberly M. Jaffe, Stephan Y. Thiberge, Margaret E. Bisher and
Rebecca D. Burdine
5. Automatic Identification of Microtubules in Cellular Electron
Tomography
Daniyar Nurgaliev, Timur Gatanov and Daniel J. Needleman
6. Imaging and analysis of the microtubule cytoskeleton in Giardia
Scott C. Dawson and Susan A. House
7. Kinetochore-microtubule dynamics and attachment stability
Jennifer G. DeLuca
8. Assessment of mitotic spindle phenotypes in Drosophila S2 cells
Gohta Goshima
9. Live cell imaging techniques for analyses of microtubules in
Dictyostelium
Matthias Samereier, Irene Meyer, Michael P. Koonce and Ralph Gräf
10. Microtubule dynamics in dendritic spines
Lukas C. Kapitein , Kah Wai Yau and Casper C. Hoogenraad
11. Analysis of Microtubules in Budding Yeast
Alexander Rauch, Elena Nazarova and Jackie Vogel
12. Determination of Microtubule Dynamic Instability in Living Cells
Kathy Kamath, Emin Oroudjev, and Mary Ann Jordan
13. Modeling microtubule-mediated forces and centrosome positioning in
Caenorhabditis elegans embryos
Akatsuki Kimura1 and Shuichi Onami
14. Cryo electron tomography of cellular microtubules
Roman I. Koning
15. Microtubule dynamics in plant cells
Henrik Buschmann, Adrian Sambade, Edouard Pesquet, Grant Calder and
Clive W. Lloyd
16. Drosophila S2 cells as a model system to investigate mitotic spindle
dynamics, architecture and function
Sara Moutinho-Pereira, Irina Matos and Helder Maiato
17. Protein micropatterns: a direct printing protocol using deep UVs
Ammar Azioune, Nicolas Carpi, Manuel Théry and Matthieu Piel
18. Optical trapping and laser ablation of microtubules in fission yeast
Nicola Maghelli and Iva M. Tolic-Nørrelykke
19. Melanophores for Microtubule dynamics and motility assays
Kazuho Ikeda, Irina Semenova, Olga Zhapparova, and Vladimir Rodionov
20. New and old reagents for fluorescent-protein tagging of microtubules
in fission yeast: experimental and critical evaluation
Hilary A. Snaith, Andreas Anders, Itaru Samejima and Kenneth E. Sawin
21. Imaging of mitotic spindle dynamics in C. elegans embryos
Mika Toya, Yumi Iida and Asako Sugimoto
22. A fast microfluidic temperature control device for studying
microtubule dynamics in fission yeast
Guilhem Velve-Casquillas, Judite Costa, Frédérique Carlier-Grynkorn ,
Adeline Mayeux and Phong T. Tran
23. Microtubule-dependent spatial organization of mitochondria in fission
yeast
Maitreyi Das, Stephan Chiron and Fulvia Verde
24. Photoactivatable GFP-Tubulin
U. Serdar Tulu*, Nick P. Ferenz
25. The Use of Fluorescence Redistribution After Photobleaching for
Analysis of Cellular Microtubule Dynamics
Claire E. Walczak, Rania S. Rizk and Sidney L. Shaw
26. Analysis of microtubule polymerization dynamics in live cells
Sarah Gierke, Praveen Kumar, and Torsten Wittmann
Ilya Grigoriev and Anna Akhmanova
2. Microscopy methods for the study of centriole biogenesis and function
in Drosophila
Ana Rodrigues Martins, Pedro Machado, Giuliano Callaini and Monica
Bettencourt-Dias
3. Quality control in single molecule studies of kinesins and
microtubule-associated proteins
Gary J. Brouhard
4. Imaging cilia in zebrafish
Kimberly M. Jaffe, Stephan Y. Thiberge, Margaret E. Bisher and
Rebecca D. Burdine
5. Automatic Identification of Microtubules in Cellular Electron
Tomography
Daniyar Nurgaliev, Timur Gatanov and Daniel J. Needleman
6. Imaging and analysis of the microtubule cytoskeleton in Giardia
Scott C. Dawson and Susan A. House
7. Kinetochore-microtubule dynamics and attachment stability
Jennifer G. DeLuca
8. Assessment of mitotic spindle phenotypes in Drosophila S2 cells
Gohta Goshima
9. Live cell imaging techniques for analyses of microtubules in
Dictyostelium
Matthias Samereier, Irene Meyer, Michael P. Koonce and Ralph Gräf
10. Microtubule dynamics in dendritic spines
Lukas C. Kapitein , Kah Wai Yau and Casper C. Hoogenraad
11. Analysis of Microtubules in Budding Yeast
Alexander Rauch, Elena Nazarova and Jackie Vogel
12. Determination of Microtubule Dynamic Instability in Living Cells
Kathy Kamath, Emin Oroudjev, and Mary Ann Jordan
13. Modeling microtubule-mediated forces and centrosome positioning in
Caenorhabditis elegans embryos
Akatsuki Kimura1 and Shuichi Onami
14. Cryo electron tomography of cellular microtubules
Roman I. Koning
15. Microtubule dynamics in plant cells
Henrik Buschmann, Adrian Sambade, Edouard Pesquet, Grant Calder and
Clive W. Lloyd
16. Drosophila S2 cells as a model system to investigate mitotic spindle
dynamics, architecture and function
Sara Moutinho-Pereira, Irina Matos and Helder Maiato
17. Protein micropatterns: a direct printing protocol using deep UVs
Ammar Azioune, Nicolas Carpi, Manuel Théry and Matthieu Piel
18. Optical trapping and laser ablation of microtubules in fission yeast
Nicola Maghelli and Iva M. Tolic-Nørrelykke
19. Melanophores for Microtubule dynamics and motility assays
Kazuho Ikeda, Irina Semenova, Olga Zhapparova, and Vladimir Rodionov
20. New and old reagents for fluorescent-protein tagging of microtubules
in fission yeast: experimental and critical evaluation
Hilary A. Snaith, Andreas Anders, Itaru Samejima and Kenneth E. Sawin
21. Imaging of mitotic spindle dynamics in C. elegans embryos
Mika Toya, Yumi Iida and Asako Sugimoto
22. A fast microfluidic temperature control device for studying
microtubule dynamics in fission yeast
Guilhem Velve-Casquillas, Judite Costa, Frédérique Carlier-Grynkorn ,
Adeline Mayeux and Phong T. Tran
23. Microtubule-dependent spatial organization of mitochondria in fission
yeast
Maitreyi Das, Stephan Chiron and Fulvia Verde
24. Photoactivatable GFP-Tubulin
U. Serdar Tulu*, Nick P. Ferenz
25. The Use of Fluorescence Redistribution After Photobleaching for
Analysis of Cellular Microtubule Dynamics
Claire E. Walczak, Rania S. Rizk and Sidney L. Shaw
26. Analysis of microtubule polymerization dynamics in live cells
Sarah Gierke, Praveen Kumar, and Torsten Wittmann