Microtubules in Cells is complemented by the companion volume by Correia that covers methods to study microtubules in vitro. The Microtubules in Cells volume includes chapters by experts around the world on many aspects of microtubule imaging in living and fixed cells; assays to study microtubule function in a wide array of model organisms and cultured cells; and high-resolution approaches to study the cytoskeleton. The authors share their years of experience, outlining potential pitfalls and critical factors to consider in experimental design, experimental implementation and data interpretation.…mehr
Microtubules in Cells is complemented by the companion volume by Correia that covers methods to study microtubules in vitro. The Microtubules in Cells volume includes chapters by experts around the world on many aspects of microtubule imaging in living and fixed cells; assays to study microtubule function in a wide array of model organisms and cultured cells; and high-resolution approaches to study the cytoskeleton. The authors share their years of experience, outlining potential pitfalls and critical factors to consider in experimental design, experimental implementation and data interpretation.
1. Microtubule dynamics at the cell cortex probed by TIRF microscopy Ilya Grigoriev and Anna Akhmanova 2. Microscopy methods for the study of centriole biogenesis and function in Drosophila Ana Rodrigues Martins, Pedro Machado, Giuliano Callaini and Monica Bettencourt-Dias 3. Quality control in single molecule studies of kinesins and microtubule-associated proteins Gary J. Brouhard 4. Imaging cilia in zebrafish Kimberly M. Jaffe, Stephan Y. Thiberge, Margaret E. Bisher and Rebecca D. Burdine 5. Automatic Identification of Microtubules in Cellular Electron Tomography Daniyar Nurgaliev, Timur Gatanov and Daniel J. Needleman 6. Imaging and analysis of the microtubule cytoskeleton in Giardia Scott C. Dawson and Susan A. House 7. Kinetochore-microtubule dynamics and attachment stability Jennifer G. DeLuca 8. Assessment of mitotic spindle phenotypes in Drosophila S2 cells Gohta Goshima 9. Live cell imaging techniques for analyses of microtubules in Dictyostelium Matthias Samereier, Irene Meyer, Michael P. Koonce and Ralph Gräf 10. Microtubule dynamics in dendritic spines Lukas C. Kapitein , Kah Wai Yau and Casper C. Hoogenraad 11. Analysis of Microtubules in Budding Yeast Alexander Rauch, Elena Nazarova and Jackie Vogel 12. Determination of Microtubule Dynamic Instability in Living Cells Kathy Kamath, Emin Oroudjev, and Mary Ann Jordan 13. Modeling microtubule-mediated forces and centrosome positioning in Caenorhabditis elegans embryos Akatsuki Kimura1 and Shuichi Onami 14. Cryo electron tomography of cellular microtubules Roman I. Koning 15. Microtubule dynamics in plant cells Henrik Buschmann, Adrian Sambade, Edouard Pesquet, Grant Calder and Clive W. Lloyd 16. Drosophila S2 cells as a model system to investigate mitotic spindle dynamics, architecture and function Sara Moutinho-Pereira, Irina Matos and Helder Maiato 17. Protein micropatterns: a direct printing protocol using deep UVs Ammar Azioune, Nicolas Carpi, Manuel Théry and Matthieu Piel 18. Optical trapping and laser ablation of microtubules in fission yeast Nicola Maghelli and Iva M. Tolic-Nørrelykke 19. Melanophores for Microtubule dynamics and motility assays Kazuho Ikeda, Irina Semenova, Olga Zhapparova, and Vladimir Rodionov 20. New and old reagents for fluorescent-protein tagging of microtubules in fission yeast: experimental and critical evaluation Hilary A. Snaith, Andreas Anders, Itaru Samejima and Kenneth E. Sawin 21. Imaging of mitotic spindle dynamics in C. elegans embryos Mika Toya, Yumi Iida and Asako Sugimoto 22. A fast microfluidic temperature control device for studying microtubule dynamics in fission yeast Guilhem Velve-Casquillas, Judite Costa, Frédérique Carlier-Grynkorn , Adeline Mayeux and Phong T. Tran 23. Microtubule-dependent spatial organization of mitochondria in fission yeast Maitreyi Das, Stephan Chiron and Fulvia Verde 24. Photoactivatable GFP-Tubulin U. Serdar Tulu*, Nick P. Ferenz 25. The Use of Fluorescence Redistribution After Photobleaching for Analysis of Cellular Microtubule Dynamics Claire E. Walczak, Rania S. Rizk and Sidney L. Shaw 26. Analysis of microtubule polymerization dynamics in live cells Sarah Gierke, Praveen Kumar, and Torsten Wittmann
1. Microtubule dynamics at the cell cortex probed by TIRF microscopy Ilya Grigoriev and Anna Akhmanova 2. Microscopy methods for the study of centriole biogenesis and function in Drosophila Ana Rodrigues Martins, Pedro Machado, Giuliano Callaini and Monica Bettencourt-Dias 3. Quality control in single molecule studies of kinesins and microtubule-associated proteins Gary J. Brouhard 4. Imaging cilia in zebrafish Kimberly M. Jaffe, Stephan Y. Thiberge, Margaret E. Bisher and Rebecca D. Burdine 5. Automatic Identification of Microtubules in Cellular Electron Tomography Daniyar Nurgaliev, Timur Gatanov and Daniel J. Needleman 6. Imaging and analysis of the microtubule cytoskeleton in Giardia Scott C. Dawson and Susan A. House 7. Kinetochore-microtubule dynamics and attachment stability Jennifer G. DeLuca 8. Assessment of mitotic spindle phenotypes in Drosophila S2 cells Gohta Goshima 9. Live cell imaging techniques for analyses of microtubules in Dictyostelium Matthias Samereier, Irene Meyer, Michael P. Koonce and Ralph Gräf 10. Microtubule dynamics in dendritic spines Lukas C. Kapitein , Kah Wai Yau and Casper C. Hoogenraad 11. Analysis of Microtubules in Budding Yeast Alexander Rauch, Elena Nazarova and Jackie Vogel 12. Determination of Microtubule Dynamic Instability in Living Cells Kathy Kamath, Emin Oroudjev, and Mary Ann Jordan 13. Modeling microtubule-mediated forces and centrosome positioning in Caenorhabditis elegans embryos Akatsuki Kimura1 and Shuichi Onami 14. Cryo electron tomography of cellular microtubules Roman I. Koning 15. Microtubule dynamics in plant cells Henrik Buschmann, Adrian Sambade, Edouard Pesquet, Grant Calder and Clive W. Lloyd 16. Drosophila S2 cells as a model system to investigate mitotic spindle dynamics, architecture and function Sara Moutinho-Pereira, Irina Matos and Helder Maiato 17. Protein micropatterns: a direct printing protocol using deep UVs Ammar Azioune, Nicolas Carpi, Manuel Théry and Matthieu Piel 18. Optical trapping and laser ablation of microtubules in fission yeast Nicola Maghelli and Iva M. Tolic-Nørrelykke 19. Melanophores for Microtubule dynamics and motility assays Kazuho Ikeda, Irina Semenova, Olga Zhapparova, and Vladimir Rodionov 20. New and old reagents for fluorescent-protein tagging of microtubules in fission yeast: experimental and critical evaluation Hilary A. Snaith, Andreas Anders, Itaru Samejima and Kenneth E. Sawin 21. Imaging of mitotic spindle dynamics in C. elegans embryos Mika Toya, Yumi Iida and Asako Sugimoto 22. A fast microfluidic temperature control device for studying microtubule dynamics in fission yeast Guilhem Velve-Casquillas, Judite Costa, Frédérique Carlier-Grynkorn , Adeline Mayeux and Phong T. Tran 23. Microtubule-dependent spatial organization of mitochondria in fission yeast Maitreyi Das, Stephan Chiron and Fulvia Verde 24. Photoactivatable GFP-Tubulin U. Serdar Tulu*, Nick P. Ferenz 25. The Use of Fluorescence Redistribution After Photobleaching for Analysis of Cellular Microtubule Dynamics Claire E. Walczak, Rania S. Rizk and Sidney L. Shaw 26. Analysis of microtubule polymerization dynamics in live cells Sarah Gierke, Praveen Kumar, and Torsten Wittmann
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