This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. Methods to assess mitochondrial function is of great interest to neuroscientists studying chronic forms of neurodegeneration, including Parkinson's, Alzheimer's, ALS, Huntington's and other triplet repeat diseases, but also to those working on acute conditions such as stroke and traumatic brain injury. This volume covers research methods on how to assess the life cycle of mitochondria including trafficking, fusion, fission, and degradation. Multiple…mehr
This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. Methods to assess mitochondrial function is of great interest to neuroscientists studying chronic forms of neurodegeneration, including Parkinson's, Alzheimer's, ALS, Huntington's and other triplet repeat diseases, but also to those working on acute conditions such as stroke and traumatic brain injury. This volume covers research methods on how to assess the life cycle of mitochondria including trafficking, fusion, fission, and degradation. Multiple perspectives on the complex and difficult problem of measurement of mitochondrial reactive oxygen species production with fluorescent indicators and techniques ranging in scope from measurements on isolated mitochondria to non-invasive imaging of metabolic function.
Anne N. Murphy, Ph.D. is currently an Associate Professor in the Department of Pharmacology within the medical school at the University of California, San Diego. She obtained her PhD from The George Washington University School of Medicine and Health Sciences in Biochemistry and Molecular Biology. Her post-doctoral studies were conducted at the Johns Hopkins Hospital and at The National Cancer Institute. Her career in both academic and biotechnology settings has focussed on mitochondrial bioenergetic function and the discovery of mitochondrial-targeted therapeutic strategies. She has particular interest in how mitochondrial metabolite and ion transport controls cell metabolism.
Inhaltsangabe
1. High-Content Functional Genomic Screening to Identify Novel Regulators of the PINK1-Parkin Pathway Andy Cheuk-Him Ng, Stephen Baird and Robert A. Screaton 2. Measurement of Mitochondrial Turnover and Life Cycle using MitoTimer Kyle M. Trudeau , Roberta A.Gottlieb and Orian S. Shirihai 3. Monitoring Mitophagy in Mammalian Cells Yushan Zhu , Guo Chen, Linbo Chen, Du Feng, Lei Liu and Quan Chen 4. Photoactivatable Green Fluorescent Protein-Based Visualization and Quantification of Mitochondrial Fusion and Mitochondrial Network Complexity in Living Cells Mariusz Karbowski, Megan M. Cleland and Brian Roelofs 5. Characterization of Mitochondrial Transport in Neurons Bing Zhou, Mei-Yao Lin, Tao Sun, Adam L. Knight and Zu-Hang Sheng 6. Imaging of Mitochondrial Dynamics in Motor and Sensory Axons of Living Mice. Irene Bolea, Wen-Biao Gan, Giovanni Manfedi and Jordi Magranél 7. Analyzing Mitochondrial Dynamics in Mouse Organotypic Slice Cultures Anh H. Pham and David C. Chan 8. Analysis of Mitochondrial Traffic in Drosophila Swathi Devireddy, Hyun Sung, Pin-Chao Liao, Elisabeth Garland-Kuntz and Peter J Hollenbeck 9. In Vivo Imaging of Mitochondria in Intact Zebrafish Larvae Dominik Paquet , Gabriela Plucinska and Thomas Misgeld 10. The Use of MiniSOG in the Localization of Mitochondrial Proteins Guy A. Perkins 11. Assessing the Function of Mitochondria-Associated ER Membranes Estela Area-Gomez. 12. Measurement of ROS Homeostasis in Isolated Mitochondria L. Tretter, and A. Ambrus 13. Use of Potentiometric Fluorophores in the Measurement of Mitochondrial Reactive Oxygen Species Brian M. Polster , David G. Nicholls , Shealinna X. Ge and Brian A. Roelofs 14. Spatial, Temporal and Quantitative Manipulation of Intracellular Hydrogen Peroxide in Cultured Cells Ishraq Alim, Renee E Haskew-Layton, Hossein Aleyasin, Hengchang Guo and Rajiv R Ratan 15. Biochemical and Biophysical Methods for Studying Mitochondrial Iron Metabolism Gregory P. Holmes-Hampton, Wing-Hang Tong and Tracey A. Rouault 16. Analysis and Interpretation of Microplate-Based Oxygen Consumption and PH Data Ajit S. Divakaruni, Alexander Paradyse1, David A. Ferrick, Anne N. Murphy ,and Martin Jastroch 17. Imaging Changes in the Cytosolic ATP-to-ADP ratio Mathew Tantama and Gary Yellen 18. The Use of MiniSOG in the Localization of Mitochondrial Proteins Carlos T. Moraes 19. Induced Pluripotent Stem Cell Derived Models for MtDNA Diseases Riikka H Hämäläinen 20. The Use of 18F-BCPP-EF as a PET Probe for Complex I Activity in the Brain Hideo Tsukada 21. MR OEF Imaging in MELAS Sheng Xie
1. High-Content Functional Genomic Screening to Identify Novel Regulators of the PINK1-Parkin Pathway Andy Cheuk-Him Ng, Stephen Baird and Robert A. Screaton 2. Measurement of Mitochondrial Turnover and Life Cycle using MitoTimer Kyle M. Trudeau , Roberta A.Gottlieb and Orian S. Shirihai 3. Monitoring Mitophagy in Mammalian Cells Yushan Zhu , Guo Chen, Linbo Chen, Du Feng, Lei Liu and Quan Chen 4. Photoactivatable Green Fluorescent Protein-Based Visualization and Quantification of Mitochondrial Fusion and Mitochondrial Network Complexity in Living Cells Mariusz Karbowski, Megan M. Cleland and Brian Roelofs 5. Characterization of Mitochondrial Transport in Neurons Bing Zhou, Mei-Yao Lin, Tao Sun, Adam L. Knight and Zu-Hang Sheng 6. Imaging of Mitochondrial Dynamics in Motor and Sensory Axons of Living Mice. Irene Bolea, Wen-Biao Gan, Giovanni Manfedi and Jordi Magranél 7. Analyzing Mitochondrial Dynamics in Mouse Organotypic Slice Cultures Anh H. Pham and David C. Chan 8. Analysis of Mitochondrial Traffic in Drosophila Swathi Devireddy, Hyun Sung, Pin-Chao Liao, Elisabeth Garland-Kuntz and Peter J Hollenbeck 9. In Vivo Imaging of Mitochondria in Intact Zebrafish Larvae Dominik Paquet , Gabriela Plucinska and Thomas Misgeld 10. The Use of MiniSOG in the Localization of Mitochondrial Proteins Guy A. Perkins 11. Assessing the Function of Mitochondria-Associated ER Membranes Estela Area-Gomez. 12. Measurement of ROS Homeostasis in Isolated Mitochondria L. Tretter, and A. Ambrus 13. Use of Potentiometric Fluorophores in the Measurement of Mitochondrial Reactive Oxygen Species Brian M. Polster , David G. Nicholls , Shealinna X. Ge and Brian A. Roelofs 14. Spatial, Temporal and Quantitative Manipulation of Intracellular Hydrogen Peroxide in Cultured Cells Ishraq Alim, Renee E Haskew-Layton, Hossein Aleyasin, Hengchang Guo and Rajiv R Ratan 15. Biochemical and Biophysical Methods for Studying Mitochondrial Iron Metabolism Gregory P. Holmes-Hampton, Wing-Hang Tong and Tracey A. Rouault 16. Analysis and Interpretation of Microplate-Based Oxygen Consumption and PH Data Ajit S. Divakaruni, Alexander Paradyse1, David A. Ferrick, Anne N. Murphy ,and Martin Jastroch 17. Imaging Changes in the Cytosolic ATP-to-ADP ratio Mathew Tantama and Gary Yellen 18. The Use of MiniSOG in the Localization of Mitochondrial Proteins Carlos T. Moraes 19. Induced Pluripotent Stem Cell Derived Models for MtDNA Diseases Riikka H Hämäläinen 20. The Use of 18F-BCPP-EF as a PET Probe for Complex I Activity in the Brain Hideo Tsukada 21. MR OEF Imaging in MELAS Sheng Xie
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