Peter Jomo Walla

Modern Biophysical Chemistry

• Broschiertes Buch

Produktbeschreibung

This updated and up-to-date version of the first edition continues with the really interesting stuff to spice up a standard biophysics and biophysical chemistry course. All relevant methods used in current cutting edge research including such recent developments as super-resolution microscopy and next-generation DNA sequencing techniques, as well as industrial applications, are explained. The text has been developed from a graduate course taught by the author for several years, and by presenting a mix of basic theory and real-life examples, he closes the gap between theory and experiment.
The first part, on basic biophysical chemistry, surveys fundamental and spectroscopic techniques as well as biomolecular properties that represent the modern standard and are also the basis for the more sophisticated technologies discussed later in the book. The second part covers the latest bioanalytical techniques such as the mentioned super-resolution and next generation sequencing methods, confocal fluorescence microscopy, light sheet microscopy, two-photon microscopy and ultrafast spectroscopy, single molecule optical, electrical and force measurements, fluorescence correlation spectroscopy, optical tweezers, quantum dots and DNA origami techniques. Both the text and illustrations have been prepared in a clear and accessible style, with extended and updated exercises (and their solutions) accompanying each chapter.
Readers with a basic understanding of biochemistry and/or biophysics will quickly gain an overview of cutting edge technology for the biophysical analysis of proteins, nucleic acids and other biomolecules and their interactions. Equally, any student contemplating a career in the chemical, pharmaceutical or bio-industry will greatly benefit from the technological knowledge presented. Questions of differing complexity testing the reader s understanding can be found at the end of each chapter with clearly described solutions available on the Wiley-VCH textbook homepage under: www.wiley-vch.de/textbooks

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Produktdetails

• Verlag: Wiley-VCH
• Artikelnr. des Verlages: 1133773 000
• 2. Aufl.
• Seitenzahl: 360
• Erscheinungstermin: 1. August 2014
• Englisch
• Abmessung: 244mm x 170mm x 19mm
• Gewicht: 717g
• ISBN-13: 9783527337736
• ISBN-10: 3527337733
• Artikelnr.: 40512019

Autorenporträt

Peter J. Walla is a group leader at the Max-Planck-Institute for Biophysical Chemistry in Göttingen (Germany), and Professor for Biophysical Chemistry at the University of Braunschweig. Having obtained his academic degrees from the Universities of Heidelberg and Göttingen, Professor Walla did postdoctoral research at the University of California at Berkeley, and headed a department in a Biotech company (now part of Bayer HealthCare) co-founded by Nobel Laureate Manfred Eigen, before taking up his current position. He has very broad research expertise in biophysical chemistry and is author of high-ranking publications covering techniques such as super-resolution microscopy, single molecule spectroscopy and two-photon ultrafast spectroscopy and their application in fields like neuroscience or photosynthetic light harvesting and energy conversion.

Inhaltsangabe

Introduction: What is Biophysical Chemistry? - An Example from Drug
Screening PART I: Basic Methods in Biophysical Chemistry BASIC OPTICAL
PRINCIPLES Introduction What Does the Electronic Structure of Molecules
Look Like? Orbitals, Wave Functions and Bonding Interactions How Does Light
Interact with Molecules? Transition Densities and the Transition Dipole
Moment Absorption Spectra of Molecules in Liquid Environments. Vibrational
Excitation and the Franck-Condon Principle What Happens After Molecules
have Absorbed Light? Fluorescence, Nonradiative Transitions and the Triplet
State Quantitative Description of all Processes: Quantum Efficiencies,
Kinetics of Excited State Populations and the Jablonski Diagram Problems
OPTICAL PROPERTIES OF BIOMOLECULES Introduction Experimental Determination
of Absorption and Fluorescence Spectra Optical Properties of Proteins and
DNA Optical Properties of Important Cofactors BASIC FLUORESCENCE TECHNQUES
Introduction Fluorescent Labelling and Linking Techniques Fluorescence
Detection Techniques Fluorscence Polarization Anisotropy Förster Resonance
Energy Transfer Fluorescence Kinetics Fluorescence Recovery after
Photobleaching Biochemiluminescence CHIROPTICAL AND SCATTERING METHODS
Chiroptical Methods Light Scattering Vibrational Spectra of Biomolecules
MAGNETIC RESONANCE TECHNIQUES Nuclear Magnetic Resonance of Biomolecules
Electron Paramagnetic Resonance MASS SPECTROMETRY Introduction MALDI-TOF
ESI-MS Structural and Sequence Analysis Using Mass Spectrometry PART II:
Advanced Methods in Biophysical Chemistry FLUORESCENCE MICROSCOPY
Introduction Conventional Fluorescence Microscopy Total Internal Reflection
Fluorescence Microscopy Light-Sheet Microscopy SUPER-RESOLUTION
FLUORESCENCE MICROSCOPY Stimulated Emission Depletion (STED) Microscopy
Photoactivated Localization Microscopy (PALM) and Stochastic Optical
Reconstruction Microscopy (STORM) 3D Super-Resolution Fluorescence
Microscopy Imaging of Live Cells Multicolour Super-Resolution Fluorescence
Microscopy Structured Illumination Microscopy SOFI Final Comparison
SINGLE-BIOMOLECULE TECHNIQUES Introduction Optical Single-Molecule
Detection Fluorescence Correlation Spectroscopy Optical Tweezers Atomic
Force Microscopy of Biomolecules Patch Clamping ULTRAFAST- AND NONLINEAR
SPECTROSCOPY Introduction Nonlinear Microscopy and Spectroscopy Ultrafast
Spectroscopy DNA SEQUENCING AND NEXT-GENERATION SEQUENCING METHODS Sanger
Method Next-Generation Sequencing Methods SPECIAL TECHNIQUES Introduction
Fluorescing Nanoparticles Surface Plasmon Resonance Detection DNA Origami
DNA Microarrays Flow Cytometry Fluorescence In Situ Hybridization
Microspheres and Nanospheres ASSAY DEVELOPMENT, READERS AND HIGH-THROUGHPUT
SCREENING Introduction Assay Development and Assay Quality Microtitre
Plates and Fluorescence Readers Application Example: Drug Discovery and
High-Throughput Screening Index