This timely book covers the need to know clinical practices for all those involved in molecular laboratory science. The field of molecular medicine is evolving at an astounding speed. Propelled by the new insights and technologies, advances are being made at an unprecedented rate. With dual measure given to today's breakthroughs, this book is a collection of the most current practices relevant to the clinical molecular laboratorian. It begins with an introductory section on techniques and procedure. It then presents four separate sections on infectious disease, oncology, pre/post-natal, and…mehr
This timely book covers the need to know clinical practices for all those involved in molecular laboratory science. The field of molecular medicine is evolving at an astounding speed. Propelled by the new insights and technologies, advances are being made at an unprecedented rate. With dual measure given to today's breakthroughs, this book is a collection of the most current practices relevant to the clinical molecular laboratorian. It begins with an introductory section on techniques and procedure. It then presents four separate sections on infectious disease, oncology, pre/post-natal, and identity testing, with specific chapters clearly outlining clinical protocols used in daily practice.
Modern Clinical Molecular Techniques cuts to the heart of what is essential for the practicing molecular laboratory scientist. It is an outstanding resource for those operating within or looking to set up a clinical molecular laboratory.
Preface.- Acknowledgement.- Contributors.- Specimen Collection, Handling and Processing.- DNA/RNA Isolation and Quantitation.- Test Validation.- In Vitro Amplification Methods in Molecular Diagnostics.-Whole Genome Sequencing in the Clinical Laboratory.-Estimation of Exon Dosage Using Real-Time Quantitative Polymerase Chain Reaction.- Reporting Clinical Molecular Genetic Laboratory Results.- HIV-1 Genotyping and Virtual Phenotyping.- COBAS® Ampliprep/Taqman HIV-1 Test.- Hepatitis C Virus (HCV) Genotype Assay (LiPA).- COBAS® Ampliprep/Taqman HCV Test.- Epstein-Barr Virus and Cytomegalovirus Viral Load Monitoring by Quantitative Real-Time Polymerase Chain Reaction.-Quantification of BK Viral Load.-Molecular Diagnosis and Epidemiology of Rabies.- Pyrosequencing for Bacterial Identification.-Other Pathogens of Significant Public Health Concern.- Gene Rearrangements Testing.-Mutation Analysis in Cancer.-Minimal Residual Disease.- Pyrosequencing in Cancer.- Clinical Applications of Microarrays in Cancer.-Basic Theories and Clinical Applications of Molecular Flow Cytometry.-Molecular Prenatal Diagnostic Testing.- PCR Based Diagnosis of Fragile X Syndrome.- Microsatellite Testing Short Tandem Repeat Profile Generation.- Parentage.-Diagnostic Molecular Training.- Molecular Diagnostics Point of Care Testing Training.
Preface.- Acknowledgement.- Contributors.- Specimen Collection, Handling and Processing.- DNA/RNA Isolation and Quantitation.- Test Validation.- In Vitro Amplification Methods in Molecular Diagnostics.-Whole Genome Sequencing in the Clinical Laboratory.-Estimation of Exon Dosage Using Real-Time Quantitative Polymerase Chain Reaction.- Reporting Clinical Molecular Genetic Laboratory Results.- HIV-1 Genotyping and Virtual Phenotyping.- COBAS® Ampliprep/Taqman HIV-1 Test.- Hepatitis C Virus (HCV) Genotype Assay (LiPA).- COBAS® Ampliprep/Taqman HCV Test.- Epstein-Barr Virus and Cytomegalovirus Viral Load Monitoring by Quantitative Real-Time Polymerase Chain Reaction.-Quantification of BK Viral Load.-Molecular Diagnosis and Epidemiology of Rabies.- Pyrosequencing for Bacterial Identification.-Other Pathogens of Significant Public Health Concern.- Gene Rearrangements Testing.-Mutation Analysis in Cancer.-Minimal Residual Disease.- Pyrosequencing in Cancer.- Clinical Applications of Microarrays in Cancer.-Basic Theories and Clinical Applications of Molecular Flow Cytometry.-Molecular Prenatal Diagnostic Testing.- PCR Based Diagnosis of Fragile X Syndrome.- Microsatellite Testing Short Tandem Repeat Profile Generation.- Parentage.-Diagnostic Molecular Training.- Molecular Diagnostics Point of Care Testing Training.
Preface.- Acknowledgement.- Contributors.- Specimen Collection, Handling and Processing.- DNA/RNA Isolation and Quantitation.- Test Validation.- In Vitro Amplification Methods in Molecular Diagnostics.-Whole Genome Sequencing in the Clinical Laboratory.-Estimation of Exon Dosage Using Real-Time Quantitative Polymerase Chain Reaction.- Reporting Clinical Molecular Genetic Laboratory Results.- HIV-1 Genotyping and Virtual Phenotyping.- COBAS® Ampliprep/Taqman HIV-1 Test.- Hepatitis C Virus (HCV) Genotype Assay (LiPA).- COBAS® Ampliprep/Taqman HCV Test.- Epstein-Barr Virus and Cytomegalovirus Viral Load Monitoring by Quantitative Real-Time Polymerase Chain Reaction.-Quantification of BK Viral Load.-Molecular Diagnosis and Epidemiology of Rabies.- Pyrosequencing for Bacterial Identification.-Other Pathogens of Significant Public Health Concern.- Gene Rearrangements Testing.-Mutation Analysis in Cancer.-Minimal Residual Disease.- Pyrosequencing in Cancer.- Clinical Applications of Microarrays in Cancer.-Basic Theories and Clinical Applications of Molecular Flow Cytometry.-Molecular Prenatal Diagnostic Testing.- PCR Based Diagnosis of Fragile X Syndrome.- Microsatellite Testing Short Tandem Repeat Profile Generation.- Parentage.-Diagnostic Molecular Training.- Molecular Diagnostics Point of Care Testing Training.
Preface.- Acknowledgement.- Contributors.- Specimen Collection, Handling and Processing.- DNA/RNA Isolation and Quantitation.- Test Validation.- In Vitro Amplification Methods in Molecular Diagnostics.-Whole Genome Sequencing in the Clinical Laboratory.-Estimation of Exon Dosage Using Real-Time Quantitative Polymerase Chain Reaction.- Reporting Clinical Molecular Genetic Laboratory Results.- HIV-1 Genotyping and Virtual Phenotyping.- COBAS® Ampliprep/Taqman HIV-1 Test.- Hepatitis C Virus (HCV) Genotype Assay (LiPA).- COBAS® Ampliprep/Taqman HCV Test.- Epstein-Barr Virus and Cytomegalovirus Viral Load Monitoring by Quantitative Real-Time Polymerase Chain Reaction.-Quantification of BK Viral Load.-Molecular Diagnosis and Epidemiology of Rabies.- Pyrosequencing for Bacterial Identification.-Other Pathogens of Significant Public Health Concern.- Gene Rearrangements Testing.-Mutation Analysis in Cancer.-Minimal Residual Disease.- Pyrosequencing in Cancer.- Clinical Applications of Microarrays in Cancer.-Basic Theories and Clinical Applications of Molecular Flow Cytometry.-Molecular Prenatal Diagnostic Testing.- PCR Based Diagnosis of Fragile X Syndrome.- Microsatellite Testing Short Tandem Repeat Profile Generation.- Parentage.-Diagnostic Molecular Training.- Molecular Diagnostics Point of Care Testing Training.
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