This manual provides an overview of the techniques used in modern neuroscience research. The emphasis is on showing how different techniques can optimally be combined in the study of problems that arise at some levels of nervous system organization. It is a working tool for the scientist in the laboratory and clinic, providing detailed step-by-step protocols with tips and recommendations. Most chapters or protocols are organized such that they can be used independently of one another. Cross-references between the chapters, a glossary, a list of suppliers and appendices provide further help.