Human parainfluenza viruses are important cause of respiratory tract diseases including lower respiratory infections which is a leading cause of deaths in infants and young children worldwide . This study was conducted to evaluate the unclear epidemiological features of Human parainfluenza viruses ( HPIVs) and their role in acute respiratory infections (ARIs) in children Iraq. Three hundred nasopharyngeal samples were collected from hospitalized pediatric patients in AL-Muthanna/ Iraq at the period from January to March /2015 and screened for HPIVs by Reverse Transcription Real Time-Polymerase Chain Reaction ( RT-PCR), specific forward ,