Toxocara canis experimental infection to laboratory animal was a good representing VLM syndrome in human.The researcher able to create molecular diagnostic equipment (kit) enable scientific laboratories works in government hospitals to detect and diagnose the early infection with T. canis parasite (larvae stage) in different paratenic tissues like sheep, poultry or cattle, local or imported meat or meat products from inside or outside Iraq. The kit consist from designed primer pairs for ITS-2 region, rrnL gene and tes-120 gene that detected T. canis adult and larvae stages are useful to provides a confirmed diagnosis with Toxocariasis in many suspected infected cases, and used routinely in government hospitals, standardization and quality control laboratories, and also in the trade ministry laboratories.