Sue Carson (North Carolina State University, Raleigh, U.S.A.), Heather B. Miller (Department of Chemistry, High Point University,, Melissa C. Srougi (High Point Assistant Professor of Biochemistry
Molecular Biology Techniques
A Classroom Laboratory Manual
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Sue Carson (North Carolina State University, Raleigh, U.S.A.), Heather B. Miller (Department of Chemistry, High Point University,, Melissa C. Srougi (High Point Assistant Professor of Biochemistry
Molecular Biology Techniques
A Classroom Laboratory Manual
- Broschiertes Buch
Presents student-tested labs proven successful in real classroom laboratories Includes a test bank on a companion website for additional testing and practice Provides exercises that simulate a cloning project that would be performed in a real research lab Includes a prep-list appendix that contains necessary recipes and catalog numbers, providing staff with detailed instructions
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Presents student-tested labs proven successful in real classroom laboratories Includes a test bank on a companion website for additional testing and practice Provides exercises that simulate a cloning project that would be performed in a real research lab Includes a prep-list appendix that contains necessary recipes and catalog numbers, providing staff with detailed instructions
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Produktdetails
- Produktdetails
- Verlag: Elsevier Science Publishing Co Inc
- 4 ed
- Seitenzahl: 294
- Erscheinungstermin: 11. März 2019
- Englisch
- Abmessung: 217mm x 275mm x 15mm
- Gewicht: 832g
- ISBN-13: 9780128180242
- ISBN-10: 0128180242
- Artikelnr.: 54589524
- Herstellerkennzeichnung
- Libri GmbH
- Europaallee 1
- 36244 Bad Hersfeld
- gpsr@libri.de
- Verlag: Elsevier Science Publishing Co Inc
- 4 ed
- Seitenzahl: 294
- Erscheinungstermin: 11. März 2019
- Englisch
- Abmessung: 217mm x 275mm x 15mm
- Gewicht: 832g
- ISBN-13: 9780128180242
- ISBN-10: 0128180242
- Artikelnr.: 54589524
- Herstellerkennzeichnung
- Libri GmbH
- Europaallee 1
- 36244 Bad Hersfeld
- gpsr@libri.de
Dr. Carson's area of scientific expertise is in molecular mechanisms of bacterial pathogenesis. She currently serves as the Director of the Master of Microbial Biotechnology Program and as a Fellow in the Office of Faculty Excellence. Prior to this appointment, she led the NC State Quality Enhancement Plan (QEP), focused on faculty development to cultivate students' critical and creative thinking skills across disciplines. During that period, she also acted as Executive Director of Academic Enrichment Programs, overseeing the Office of Undergraduate Research, Fellowship Advising, and the University Honors and Scholars Programs. Dr. Carson spent over ten years leading curriculum development for the NC State Biotechnology Program. She has received multiple awards for teaching excellence and innovation and was a member of the Howard Hughes Science Education Alliance, promoting and implementing inquiry-guided learning and authentic research in undergraduate curricula. She authored three molecular biology lab manuals and has published numerous peer-reviewed papers in the area of course and curriculum development. She has mentored over 100 undergraduate students in research projects and was the PI and Director of the National Science Foundation (NSF)-funded Integrative Plant and Microbial Systems Research Experience for Undergraduates (REU) Program for over a decade. Most recently, she completed a 2-year rotation at the National Science Foundation in the Directorate for Education and Human Resources. Within the Triangle community, she has served on the Board of Directors of the Wake County Beekeeping Association and the Triangle Swing Dance Society.
Part I: Manipulation of DNAAdvanced Alternatives Within Part I 1. Getting
Oriented; Practicing With Micropipettes 2. Purification and Digestion of
Plasmid (Vector) DNA3. Completion of Vector Preparation and Polymerase
Chain Reaction Amplification of egfp 4. Preparation of Insert DNA (egfp)
PCR Product5. DNA Ligation and Transformation of Escherichia coli
Part II: Screening TransformantsAdvanced Alternatives Within Part II6.
Screening of Transformants, Part I 7. Screening of Transformants, Part II8.
Analysis of DNA Sequence From a Positive Clone, Part II
Part III: Expression, Detection, and Purification of Recombinant Proteins
from BacteriaAdvanced Alternatives Within Part III 9. Expression of Fusion
Protein from Positive Clones, SDS_PAGE and Western Blot: Part I 10.
Expression of Fusion Protein from Positive Clones, SDS_PAGE, and Western
Blot: Part II 11. Extraction of Recombinant Protein From Escherichia coli
Using a Glutathione Affinity Column12. Analysis of Purification Fractions
Part IV: Analysis of mRNA Levels13. Total RNA Purification14. Analysis of
gst::egfp mRNA Levels by RT-qPCR: Part I15. Analysis of gst::egfp mRNA
Levels by RT-qPCR: Part II 16. Analysis of gst::egfp mRNA Levels by
Semiquantitative RT-PCR: Part I 17. Analysis of gst::egfp mRNA Levels by
Semiquantitative RT-PCR: Part II
Part V: Modulation of Gene Expression18. Culturing Mammalian Cells19.
Transient Transfection of Mammalian Cells20. RNAi-Mediated Knockdown of
EGFP: Part I 21. RNAi-Mediated Knockdown of EGFP: Part II22. RNAi-Mediated
Knockdown of EGFP: Part III23. CRISPR-Mediated Knockout of EGFP: Part I24.
CRISPR-Mediated Knockout of EGFP: Part II 25. Advanced CRISPR: Part I 26.
Advanced CRISPR: Part II27. Interim Laboratory Session 28. Advanced CRISPR:
Part IV 29. Advanced CRISPR: Part V30. Advanced CRISPR: Part VI
AppendixA: Equipment B: Prep List C: Preparation of Competent Escherichia
coli Cells D: Pre-Lab Questions
Oriented; Practicing With Micropipettes 2. Purification and Digestion of
Plasmid (Vector) DNA3. Completion of Vector Preparation and Polymerase
Chain Reaction Amplification of egfp 4. Preparation of Insert DNA (egfp)
PCR Product5. DNA Ligation and Transformation of Escherichia coli
Part II: Screening TransformantsAdvanced Alternatives Within Part II6.
Screening of Transformants, Part I 7. Screening of Transformants, Part II8.
Analysis of DNA Sequence From a Positive Clone, Part II
Part III: Expression, Detection, and Purification of Recombinant Proteins
from BacteriaAdvanced Alternatives Within Part III 9. Expression of Fusion
Protein from Positive Clones, SDS_PAGE and Western Blot: Part I 10.
Expression of Fusion Protein from Positive Clones, SDS_PAGE, and Western
Blot: Part II 11. Extraction of Recombinant Protein From Escherichia coli
Using a Glutathione Affinity Column12. Analysis of Purification Fractions
Part IV: Analysis of mRNA Levels13. Total RNA Purification14. Analysis of
gst::egfp mRNA Levels by RT-qPCR: Part I15. Analysis of gst::egfp mRNA
Levels by RT-qPCR: Part II 16. Analysis of gst::egfp mRNA Levels by
Semiquantitative RT-PCR: Part I 17. Analysis of gst::egfp mRNA Levels by
Semiquantitative RT-PCR: Part II
Part V: Modulation of Gene Expression18. Culturing Mammalian Cells19.
Transient Transfection of Mammalian Cells20. RNAi-Mediated Knockdown of
EGFP: Part I 21. RNAi-Mediated Knockdown of EGFP: Part II22. RNAi-Mediated
Knockdown of EGFP: Part III23. CRISPR-Mediated Knockout of EGFP: Part I24.
CRISPR-Mediated Knockout of EGFP: Part II 25. Advanced CRISPR: Part I 26.
Advanced CRISPR: Part II27. Interim Laboratory Session 28. Advanced CRISPR:
Part IV 29. Advanced CRISPR: Part V30. Advanced CRISPR: Part VI
AppendixA: Equipment B: Prep List C: Preparation of Competent Escherichia
coli Cells D: Pre-Lab Questions
Part I: Manipulation of DNAAdvanced Alternatives Within Part I 1. Getting
Oriented; Practicing With Micropipettes 2. Purification and Digestion of
Plasmid (Vector) DNA3. Completion of Vector Preparation and Polymerase
Chain Reaction Amplification of egfp 4. Preparation of Insert DNA (egfp)
PCR Product5. DNA Ligation and Transformation of Escherichia coli
Part II: Screening TransformantsAdvanced Alternatives Within Part II6.
Screening of Transformants, Part I 7. Screening of Transformants, Part II8.
Analysis of DNA Sequence From a Positive Clone, Part II
Part III: Expression, Detection, and Purification of Recombinant Proteins
from BacteriaAdvanced Alternatives Within Part III 9. Expression of Fusion
Protein from Positive Clones, SDS_PAGE and Western Blot: Part I 10.
Expression of Fusion Protein from Positive Clones, SDS_PAGE, and Western
Blot: Part II 11. Extraction of Recombinant Protein From Escherichia coli
Using a Glutathione Affinity Column12. Analysis of Purification Fractions
Part IV: Analysis of mRNA Levels13. Total RNA Purification14. Analysis of
gst::egfp mRNA Levels by RT-qPCR: Part I15. Analysis of gst::egfp mRNA
Levels by RT-qPCR: Part II 16. Analysis of gst::egfp mRNA Levels by
Semiquantitative RT-PCR: Part I 17. Analysis of gst::egfp mRNA Levels by
Semiquantitative RT-PCR: Part II
Part V: Modulation of Gene Expression18. Culturing Mammalian Cells19.
Transient Transfection of Mammalian Cells20. RNAi-Mediated Knockdown of
EGFP: Part I 21. RNAi-Mediated Knockdown of EGFP: Part II22. RNAi-Mediated
Knockdown of EGFP: Part III23. CRISPR-Mediated Knockout of EGFP: Part I24.
CRISPR-Mediated Knockout of EGFP: Part II 25. Advanced CRISPR: Part I 26.
Advanced CRISPR: Part II27. Interim Laboratory Session 28. Advanced CRISPR:
Part IV 29. Advanced CRISPR: Part V30. Advanced CRISPR: Part VI
AppendixA: Equipment B: Prep List C: Preparation of Competent Escherichia
coli Cells D: Pre-Lab Questions
Oriented; Practicing With Micropipettes 2. Purification and Digestion of
Plasmid (Vector) DNA3. Completion of Vector Preparation and Polymerase
Chain Reaction Amplification of egfp 4. Preparation of Insert DNA (egfp)
PCR Product5. DNA Ligation and Transformation of Escherichia coli
Part II: Screening TransformantsAdvanced Alternatives Within Part II6.
Screening of Transformants, Part I 7. Screening of Transformants, Part II8.
Analysis of DNA Sequence From a Positive Clone, Part II
Part III: Expression, Detection, and Purification of Recombinant Proteins
from BacteriaAdvanced Alternatives Within Part III 9. Expression of Fusion
Protein from Positive Clones, SDS_PAGE and Western Blot: Part I 10.
Expression of Fusion Protein from Positive Clones, SDS_PAGE, and Western
Blot: Part II 11. Extraction of Recombinant Protein From Escherichia coli
Using a Glutathione Affinity Column12. Analysis of Purification Fractions
Part IV: Analysis of mRNA Levels13. Total RNA Purification14. Analysis of
gst::egfp mRNA Levels by RT-qPCR: Part I15. Analysis of gst::egfp mRNA
Levels by RT-qPCR: Part II 16. Analysis of gst::egfp mRNA Levels by
Semiquantitative RT-PCR: Part I 17. Analysis of gst::egfp mRNA Levels by
Semiquantitative RT-PCR: Part II
Part V: Modulation of Gene Expression18. Culturing Mammalian Cells19.
Transient Transfection of Mammalian Cells20. RNAi-Mediated Knockdown of
EGFP: Part I 21. RNAi-Mediated Knockdown of EGFP: Part II22. RNAi-Mediated
Knockdown of EGFP: Part III23. CRISPR-Mediated Knockout of EGFP: Part I24.
CRISPR-Mediated Knockout of EGFP: Part II 25. Advanced CRISPR: Part I 26.
Advanced CRISPR: Part II27. Interim Laboratory Session 28. Advanced CRISPR:
Part IV 29. Advanced CRISPR: Part V30. Advanced CRISPR: Part VI
AppendixA: Equipment B: Prep List C: Preparation of Competent Escherichia
coli Cells D: Pre-Lab Questions