Soybean is one of the most popular protein rich leguminous crop used as food and feed. However, yield performance is very low and varied in different environments drastically in Bangladesh. Therefore, the present work has been planned to select high yielded stable soybean genotypes through mutation breeding. I performed two experiments where fifteen soybean genotypes were used. In the first experiment the superior soybean mutant genotypes were selected by testing yield performance and genotype-environment interaction was observed for stability. In the second experiment selected soybean mutants were characterized using RAPD marker. Polymorphic bands of RAPD markers suggesting that genotypes were different from each other and possessed genetic diversity among them. Thus, these markers could be efficient for comparing the genetic relationships, patterns of variation and measurement of genetic distance among the selected soybean genotypes. Therefore, from these soybean mutants SBM-09, SBM-17 and BINA Soybean-1 had been selected as stable and higher yield performing genotypes for enhancing yield.