Molecular Cytogenetics

Protocols and Applications
Herausgegeben:Fan, Yao-Shan

• Broschiertes Buch

Produktbeschreibung

The new techniques of molecular cytogenetics, mainly fluorescence in situ hybridization (FISH) of DNA probes to metaphase chromosomes or interphase nuclei, have been developed in the past two decades. Many FISH techniques have been implemented for diagnostic services, whereas some others are mainly used for investigational purposes. Several hundreds of FISH probes and hybridization kits are now commercially available, and the list is growing rapidly. FISH has been widely used as a powerful diagnostic tool in many areas of medicine including pediatrics, medical genetics, maternal-fetal medicine, reproductive medicine, pathology, hematology, and oncology. Frequently, a physician may be puzzled by the variety of FISH techniques and wonder what test to order. It is not uncommon that a sample is referred to a laboratory for FISH without indicating a specific test. On the other hand, a cytogeneticist or a technologist in a laboratory needs, from case to case, to determine which procedure to perform and which probe to use for an informative result. To obtain the best results, one must use the right DNA probes and have reliable protocols and measures of quality assurance in place. Also, one must have sufficient knowledge in both traditional and molecular cytogenetics, as well as the particular areas of medicine for which the test is used in order to appropriately interpret the FISH results, and to correlate them with clinical diagnosis, treatment, and prognosis.

Produktdetails

• Methods in Molecular Biology 204
• Verlag: Humana / Humana Press / Springer, Berlin
• Artikelnr. des Verlages: 978-1-61737-300-8
• Softcover reprint of hardcover 1st ed. 2003
• Seitenzahl: 414
• Erscheinungstermin: 10. November 2010
• Englisch
• Abmessung: 228mm x 151mm x 32mm
• Gewicht: 611g
• ISBN-13: 9781617373008
• ISBN-10: 1617373001
• Artikelnr.: 32201546

Inhaltsangabe

Basic Concepts and Techniques.- Molecular Cytogenetics in Medicine.- Labeling Fluorescence In Situ Hybridization Probes for Genomic Targets.- Labeling Fluorescence In Situ Hybridization Probes for RNA Targets.- Basic FISH Techniques and Troubleshooting.- Evolving Techniques and Applications.- Chromosome Microdissection.- Primed In Situ Labeling.- Spectral Karyotyping.- Multicolor FISH.- Characterization of Constitutional Chromosome Abnormalities by Comparative Genomic Hybridization.- Color Banding.- Multicolor Fiber FISH.- Multi-Telomere FISH.- Fluorescence Genotyping for Screening Cryptic Telomeric Rearrangements.- Microarray CGH.- Special Applications in Chromosomal Disorders.- Molecular Approaches for Delineating Marker Chromosomes.- Interphase FISH for Prenatal Diagnosis of Common Aneuploidies.- Prenatal Diagnosis of Chromosomal Abnormalities Using Maternal Blood.- Preimplantation FISH Diagnosis of Aneuploidies.- Microdeletion Syndromes.- Molecular Detection of Uniparental Disomy.- Molecular Cytogenetics in Reproductive Pathology.- Special Applications in Oncology.- Interphase FISH Studies of Chronic Myeloid Leukemia.- Chromogenic In Situ Hybridization and FISH in Pathology.- FISH Detection of HER2 Amplification in Breast Cancer.- Comparative Genomic Hybridization in Cancer Investigations.- Simultaneous Fluorescence Immunophenotyping and FISH on Tumor Cells.- BAC Resource for Molecular Cytogenetics.

Rezensionen

"Dr. Fan has done a marvelous job of putting together a collection of laboratory protocols and review articles of different FISH technologies...Molecular Cytogenetics: Protocols and Applications is packed with information on cutting-edge FISH technologies and their applications, limitations, and pitfalls, along with detailed laboratory protocols. I would certainly recommend that this book be included in the libraries of cytogeneticists and cytogenetic laboratories" - Am. J. Hum. Gent.

"Dr. Fan has done a marvelous job of putting together a collection of laboratory protocols and review articles of different FISH technologies. The authors of the different chapters not only gave detailed, easy-to-follow protocols, but also provided their first-hand experiences of troubleshooting. Molecular Cytogenetics: Protocols and Applications is packed with information on cutting-edge FISH technologies and their applications, limitations, and pitfalls, along with detailed laboratory protocols. I would certainly recommend that this book be included in the libraries of cytogeneticists and cytogenetic laboratories." -The American Society of Human Genetics