Molecular diagnosis of Bovine Trypanosomosis using LAMP

Molecular diagnosis of Bovine Trypanosomosis using LAMP

Comparison of the use of Microscopy and LAMP in the Determination of the Epidemiology of the Bovine Trypanosomosis

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Over the past years, use of microscopy in the determination of bovine trypanosome infections has yielded important information on the epidemiology of the disease. However, recent molecular techniques such as Loop-mediated isothermal amplification (LAMP) have been developed. LAMP technique, specifically targets the 18S rRNA gene of Trypanosoma congolense, the repetitive insertion mobile element (RIME) gene of the Trypanozoon subgenus group and the human serum resistant associated (SRA) gene of Trypanosoma brucei rhodesiense. LAMP is a highly sensitive and specific technique and yet user friendl...