This a comprehensive work introduced by the author to distinguish between eight Egyptian barley cultivars (Hordeum vulgare L) with respect to their tolerance against salt stress and to detect physiological, biochemical and molecular genetic markers for this character. Nine yield traits and its attributes were scored to detect the most four salt-tolerant and the most four salt-sensitive cultivars for subsequent physiological, biochemical and molecular genetic studies. Correlation between germination percentage, germination rate and abscisic acid content and salt stress were investigated. SDS protein profiles indicated the occurrence of a number of biochemical markers in which some of them were positively associated with salt tolerance, while the others were negatively associated. On the molecular level, RAPD analysis was done utilizing eighteen 10-mer random primers. The results showed the occurrence of some molecular genetic markers associated with salt tolerance. In conclusion, these different markers would be useful in screening different cultivars for their tolerance against salt stress during breeding programs of barley.