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The Fifth -and last- Supplement expands the existing sections on: 3: Identification and classification of microbes using DNA and RNA sequences, 8: Molecular tools to assess microbial activities. The Molecular Microbial Ecology Manual provides general information about molecular techniques relevant to molecular ecological research. Attention is paid to the use of molecular methods for studying the fate of genetically modified and native microorganisms in the environment. Audience: Postgraduate students and research scientists in the plant and soils sciences and plant molecular biology.