Over recent decades, parasitic have been recognized as having great potential to cause food borne diseases. The organisms of greatest concern in food production worldwide are Cryptosporidium spp. and Cyclospora spp. Although other parasitic can be spread by food, current epidemiological evidence suggests that present the largest risks. Milk products are one vector food dangerous for bacteria intestinal coliform Escherichia coli that has become a serious problem threatening public health for being responsible events have multiple instances of food poisoning accompanied by watery diarrhea that becomes bloody diarrhea and cause is a case hemorrhagic colitis. This book therefore, provides a new metric of success for detection of contamination rate in buffalo milk products and reduce the incidence of food borne disease, watery diarrhea, bloody diarrhea, hemorrhagic colitis through the book collected the specimens, Culture, Biochemical tests, and PCR identification of Escherichia coli,Klebsiella pneumonia, Cryptosporidium spp. and Cyclospora spp. was done to exact diagnosis of these microorganisms in buffalo milk products.