Monoclonal Antibodies

The Second Generation
Herausgeber: Zola, Heddy

• Gebundenes Buch

Produktbeschreibung

A review of what needs to be done to realise the potential of monoclonal antibodies. The book assesses the competing technologies with advice on the best approach for a particular situation.

Produktdetails

• Verlag: Routledge
• Seitenzahl: 220
• Erscheinungstermin: 26. Juni 1995
• Englisch
• Abmessung: 234mm x 156mm x 14mm
• Gewicht: 490g
• ISBN-13: 9781872748788
• ISBN-10: 1872748783
• Artikelnr.: 21593174

Autorenporträt

Chief Hospital Scientist and Professor of Immunology, Flinders Medical Centre and Flinders University, Adelaide, Australia.

Inhaltsangabe

Contributors
Abbreviations
Preface
Safety note
1. Introduction. H. Zola
Introduction: the purpose of this book
Monoclonal antibodies: scope and limitations
Antibodies as laboratory reagents: a brief perspective
Antibodies as therapeutic agents: a brief perspective
Conclusions
References
2. Making and using 'conventional' mouse monoclonal antibodies. P.J. Donohoe, P.J. Macardle and H. Zola
Introduction
Tissue culture
Materials
Working solutions
The screening assay
Immunization
The myeloma cell line
Fusion protocol
Feeder cells
Post
fusion care
Mycoplasma
Cloning
Cryopreservation
Thawing cells out
Ascites fluid
Production of monoclonal antibodies in dialysis tubing
Continuous perfusion culture systems
Purification
Conjugation
References
3. Human monoclonal antibodies from immortalized B cells. A. W. Hohmann, L. Spatz, M. Irigoyen and A. Manheimer
Lory
Introduction
Immortalized B cells to probe the human antibody repertoire
Infectious diseases
Human immunodeficiency virus
Erythrocyte antigens
Human histocompatibility antigens
Tumor antigens
Autoantibodies
Immortalization of B cells with EBV
EBV transformation of B cells: methodology
Making human hybridomas
Hybridoma methodology
References
4. Reconstruction of monoclonal antibodies by genetic engineering. J.R. Adair and D.J. King
Introduction
The reconstruction process
The binding site
The effector functions
Reconstructed antibodies in practice
Summary and conclusions
References
5. The genetic engineering of antibody constructs for diagnosis and therapy. G.A. Pietersz and l.F.C. McKenzie
Introduction
Principles of use of antibody conjugates
Recent advances in immunoconjugate construction
Recombinant antibodies and fusion proteins
Use of cytokines and growth factors for targeting
Potential problems
Conclusions
References
6. Superseding hybridoma technology with phage display libraries. R. Irving and P. Hudson
Introduction
Affinity maturation
Discussion
References
7. Construction and application of libraries of artificial antibodies. W. Ito and Y. Kurosawa
Introduction
Expression in E. coli of antibodies whose V genes are derived from V" and V L genes expressed in animals
Characteristics of phage display antibodies
Various devices for the generation of artificial antibodies
Construction of libraries of completely synthetic antibodies
Application of libraries of artificial antibodies
Future perspectives
References
8. Expression of antibody genes in mammalian cells. C. Bebbington
Introduction
Transient expression
Stable mammalian expression systems
Other factors influencing expression in mammalian cells
Choosing an expression system
References
9. Structure and application of single
chain Fvs as diagnostic and therapeutic agents. P. Hudson
Introduction
Design of single
chain Fv fragments
Construction of scFv genes
The structure of scFv fragments
The design of fusion proteins
The design of bifunctional scFvs ( diabodies)
The choice of expression systems
Scale
up production of scFvs in fermenters
Case studies: the design and expression of scFv molecules (for crystal structures and novel diagnostics)
Applications of scFvs in diagnostics
Discussion
References
Index.