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Bacteria resistance to antibiotics has become a worldwide problem impacted partly by antibiotics used in livestock production. Multiple antibiotic resistant bacteria pose a major threat to the successful therapeutic use of antibiotics in human and veterinary medicine. This study, therefore, is aimed at determining the resistance pattern of bacteria isolated from pig dung.The study investigated the pattern of antibiotic resistance in bacteria species isolated from pig dung from five piggeries in Ogbomoso, Southwestern Nigeria. Thirty pig dung samples were collected from each of the piggeries. Bacteria were isolated from the samples on Eosin Methylene Blue and characterized by standard laboratory methods. Further molecular identification of the multi-drug resistant isolates was carried out using 16S rRNA. A total of 113 bacteria isolates were tested against selected antibiotics using disk diffusion method. The isolates showed the highest resistance to cefixime (97.35%), ceftazidime(29.20%), ceftriaxone and cefuroxime (24.78%), nitrofurantoin (18.58%), gentamicin (15.93%), augmentin (14.16%), ofloxacin and ciprofloxacin (13.27%) while cefepime (5.31%) had the lowest resistance.