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Genetic defects are very common in every population such as orofacial clefts that affect the normal facial anatomy. MSX1 is one of the genes controlling the facial features. This book will be helpful for the students who are just starting their Genetic studies at basic level. More over this book has various protocols involved in molecular examinations including DNA Isolation, Primer designing, Polymerase Chain Reaction and introduction of various elution kits. Students can also go through different bioinformatics tools involved in mutation analysis. In addition you can find some information on basic structures responsible for normal facial development. This study was conducted for the mutation analysis of MSX1 that comprised of two exons. The part we evaluated was not actually responsible for the clefts in our sample population. But mutations in this gene (MSX1) has played critical roles in some other populations which is very obviously reported by different scientists throughoutthe world.