This standard operating protocol (SOP) manual is a guideline intended to assist as a reference material for routine laboratory activities in mycology laboratory. The design and content of this manual provides an introductory information and guideline in mycology laboratory as well as a techniques for a better understanding for advanced in fungal research. The manual is especially useful for biologists, mycologist, microbiologist, plant pathologists, laboratory technologists, environmentalists and particularly to those who are willing to pursue a career in mycology or engage in research and advanced fungal studies.Generally this manual is intended to introduce the techniques that are used in specimen collection, isolation, screening, purification, identification and characterization techniques of fungi in microbiology laboratory for fungal diversity study and conservation of genetic resource at microbial Gene Bank.