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AM fungi are an important component in the soil microbial mass which regulates several essential biological processes at the plant-soil interface. The majority of higher green plants and a large number of AM fungi are involved in mycorrhiza formation. The rhizosphere soil samples and roots of 20 medicinal plants were examined for AM fungal association and maximum spore population was observed in Mirabilis jalapa and minimum spore population was observed in Crotalaria pallida. A total of 24 AM fungal spores were isolated from these medicinal plant species and all the selected plants showed VAM fungal association.